Human Fibrillin-3(FBN3) ELISA kit

Code CSB-EL008458HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
fibrillin 3
Alternative Names
FBN3 ELISA Kit; KIAA1776Fibrillin-3 [Cleaved into: Fibrillin-3 C-terminal peptide] ELISA Kit
Abbreviation
FBN3
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FBN3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %93-99
1:2Average %94
Range %89-98
1:4Average %91
Range %88-98
1:8Average %86
Range %80-96
Recovery
The recovery of human FBN3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8781-93
EDTA plasma (n=4)9895-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.409 2.456 2.433 2.325
101.535 1.585 1.560 1.452
50.812 0.801 0.807 0.699
2.50.506 0.545 0.526 0.418
1.250.272 0.265 0.269 0.161
0.6250.166 0.155 0.161 0.053
0.3120.150 0.158 0.154 0.046
00.104 0.111 0.108  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human FBN3 ELISA Kit was designed for the quantitative measurement of Human FBN3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

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Target Background

Function
(From Uniprot)
Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-containing microfibrils provide long-term force bearing structural support.
Gene References into Functions
  1. an association study showed a potential association of the D19S884 marker with PCOS in Chinese Han women and the meta-analysis identified that the A8 allele may increase susceptibility to PCOS PMID: 23265956
  2. The FBN3 risk allele may be associated with changes in basal glucose homeostasis in PCOS. PMID: 22301903
  3. Data show that TGF-beta pathways operate during ovarian fetal development, and fibrillin 3 is highly expressed at a critical stage early in developing human and bovine fetal ovaries. PMID: 21411746
  4. fibrillin-3, compared to the other fibrillins, fulfills both overlapping and distinct functions in human development PMID: 20970500
  5. A variant in the fibrillin-3 gene is associated with TGF-beta and inhibin B levels in women with polycystic ovary syndrome. PMID: 20630504
  6. Loss of fibrillin-3 during folliculogenesis may be an important factor in polycystic ovary syndrome pathogenesis. PMID: 20855553
  7. Single nucleotide polymorphisms in FBN3 occur and may be a candidate gene for polycystic ovary syndrome. PMID: 20200332
  8. Data show that fibrillin 3 appears to have little involvement in PCOS but cannot rule out that other markers in the region of chromosome 19p13.2 are associated with PCOS or that FBN3 expression occurs in other organs that may influence the PCOS phenotype. PMID: 19692420
  9. fibrillin-3 gene showed the strongest evidence for association with Polycystic ovary syndrome PMID: 17785364

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Fibrillin family
Tissue Specificity
Predominantly expressed in connective tissues such as skeletal muscle, tendon, skin, perichondrium and periosteum. Highly expressed in fetal lung, brain, kidney. Expressed at low level in prostate, testis, mammary gland, uterus, ovary, placenta, bladder,
Database Links

HGNC: 18794

OMIM: 608529

KEGG: hsa:84467

STRING: 9606.ENSP00000270509

UniGene: Hs.370362

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