Human Fibrinogen(FB) ELISA Kit

Code CSB-E13656h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Fibrinogen(FB)
Alternative Names N/A
Abbreviation Fb
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 μg/mL-20 μg/mL
Sensitivity 0.078 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human FB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:100Average %85
Range %80-92
1:200Average %98
Range %90-105
1:400Average %97
Range %90-105
1:800Average %93
Range %86-98
The recovery of human FB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-98
EDTA plasma (n=4)9890-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
202.648 2.701 2.675 2.583
102.159 2.169 2.164 2.072
51.476 1.536 1.506 1.414
2.50.864 0.876 0.870 0.778
1.250.443 0.475 0.459 0.367
0.6250.234 0.253 0.244 0.152
0.3120.146 0.154 0.150 0.058
00.095 0.089 0.092  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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