Code | CSB-EL008624HU |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human FGF19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 92 | |||||
Range % | 87-99 | ||||||
1:2 | Average % | 103 | |||||
Range % | 99-104 | ||||||
1:4 | Average % | 93 | |||||
Range % | 87-98 | ||||||
1:8 | Average % | 91 | |||||
Range % | 89-97 |
The recovery of human FGF19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 95 | 92-99 | |||||
EDTA plasma (n=4) | 90 | 86-93 | |||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human FGF19 ELISA Kit is used to quantitatively measure human FGF19 levels in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of FGF19 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
FGF19 is an ileum-derived postprandial enterokine that governs bile acid and nutrient metabolism. FGF19 mediates the negative feedback regulation of bile salt synthesis by bile salts. FGF19 is inducibly expressed by the terminal ileum in response to bile acids that are reabsorbed from the intestine. It is released to the portal blood and will bind to its receptor in the liver to down-regulate the rate-limiting enzyme in bile acid synthesis. β-Klotho enables FGF19 binding to FGFR4 and acts as the obligatory coreceptor that permits FGF19–FGFR4 signaling.
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