Human Free Testosterone,F-TESTO ELISA Kit

Human Free Testosterone,F-TESTO ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Human Free Testosterone,F-TESTO ELISA Kit

Citations (7) Protocol

Code	CSB-E05096h
Size	96T,5×96T,10×96T
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Product Details
Citations (7)
Related Products
Customer Reviews and Q&A

Target Name

Free Testosterone,F-TESTO

Abbreviation

F-TESTO

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

7.5 pg/mL-1500 pg/mL

Sensitivity

3.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	94
	Range %	91-98
1:4	Average %	97
	Range %	93-101
1:8	Average %	94
	Range %	89-98

Recovery

The recovery of human F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	105	101-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1500	0.174	0.176	0.175
375	0.324	0.338	0.331
120	0.535	0.514	0.525
30	0.812	0.854	0.833
7.5	1.421	1.334	1.378
0	1.987	1.912	1.950

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human F-TESTO ELISA Kit was designed for the quantitative measurement of Human F-TESTO protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 7.5 pg/mL-1500 pg/mL and the sensitivity is 3.75 pg/mL.

The correlation between serum fructose levels and pregnancy outcomes in IVF patients with and without PCOS: a case-control study B Zhang, Y Li, Y Li, J Song, Y Fang,BMC Pregnancy and Childbirth,2025
Impact of diet and physical activity on reproductive profile: A comparison of middle-aged and elderly men. H Ashraf,/,2024
Serum fructose levels are positively correlated with dyslipidemia in women with polycystic ovary syndrome Z Na,Reproductive BioMedicine Online,2023
Antioxidant mitoquinone suppresses benign prostatic hyperplasia by regulating the AR–NLRP3 pathway BR Jin,Redox biology,2023
Fructose-associated elevation of serum uric acid levels may be involved in metabolic disorders in polycystic ovary syndrome: A case-control study D Feng,Authorea Preprints,2022
Association of galactose and insulin resistance in polycystic ovary syndrome: A case-control study Z Na,eClinicalMedicine,2022
Fructose levels are elevated in women with polycystic ovary syndrome with obesity and hyperinsulinemia Shi B,Hum Reprod,2020

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.