| Description |
This Human F-TESTO ELISA Kit was designed for the quantitative measurement of Human F-TESTO protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 7.5 pg/mL-1500 pg/mL and the sensitivity is 3.75 pg/mL. |
| Target Name |
Free Testosterone,F-TESTO |
| Abbreviation |
F-TESTO |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
7.5 pg/mL-1500 pg/mL |
| Sensitivity |
3.75 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Epigenetics and Nuclear Signaling
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| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
|
| Range % |
92-102 |
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| 1:2 |
Average % |
94 |
|
| Range % |
91-98 |
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| 1:4 |
Average % |
97 |
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| Range % |
93-101 |
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| 1:8 |
Average % |
94 |
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| Range % |
89-98 |
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| Recovery |
| The recovery of human F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
98 |
92-104 |
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| EDTA plasma (n=4) |
105 |
101-109 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml |
OD1 |
OD2 |
Average |
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| 1500 |
0.174 |
0.176 |
0.175 |
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| 375 |
0.324 |
0.338 |
0.331 |
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| 120 |
0.535 |
0.514 |
0.525 |
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| 30 |
0.812 |
0.854 |
0.833 |
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| 7.5 |
1.421 |
1.334 |
1.378 |
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| 0 |
1.987 |
1.912 |
1.950 |
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
