Human Free Testosterone,F-TESTO ELISA Kit

Code CSB-E05096h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details


This Human F-TESTO ELISA Kit was designed for the quantitative measurement of Human F-TESTO protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 7.5 pg/mL-1500 pg/mL and the sensitivity is 3.75 pg/mL.

Target Name Free Testosterone,F-TESTO
Abbreviation F-TESTO
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 7.5 pg/mL-1500 pg/mL
Sensitivity 3.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 92-102  
1:2 Average % 94  
Range % 91-98  
1:4 Average % 97  
Range % 93-101  
1:8 Average % 94  
Range % 89-98  
The recovery of human F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 92-104  
EDTA plasma (n=4) 105 101-109  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1500 0.174 0.176 0.175    
375 0.324 0.338 0.331    
120 0.535 0.514 0.525    
30 0.812 0.854 0.833    
7.5 1.421 1.334 1.378    
0 1.987 1.912 1.950    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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