Rat Free Testosterone,F-TESTO ELISA Kit

Code CSB-E05097r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat F-TESTO ELISA Kit was designed for the quantitative measurement of Rat F-TESTO protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.3 pg/mL-60 pg/mL and the sensitivity is 0.15 pg/mL.

Target Name Free Testosterone,F-TESTO
Abbreviation F-TESTO
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 0.3 pg/mL-60 pg/mL
Sensitivity 0.15 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 90-101  
1:2 Average % 95  
Range % 89-99  
1:4 Average % 94  
Range % 90-98  
1:8 Average % 95  
Range % 90-100  
Recovery
The recovery of rat F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 89-104  
EDTA plasma (n=4) 94 88-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
60 0.202 0.205 0.204    
15 0.359 0.342 0.351    
4.8 0.610 0.623 0.617    
1.2 1.007 1.012 1.010    
0.3 1.464 1.457 1.461    
0 2.439 2.454 2.447    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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