Human Galanin-like peptide(GALP) ELISA kit

Code CSB-EL009222HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9UBC7
Lead Time 7-14 working days
Abbreviation GALP
Target Name galanin-like peptide
Alias , alarin|gal-like peptide
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 24840
RGD 620187
MGI 2663979
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %87-97
1:2Average %101
Range %97-103
1:4Average %95
Range %88-98
1:8Average %99
Range %94-102
Recovery
The recovery of human GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-102
EDTA plasma (n=4)9488-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.057 2.080 2.069 1.918
51.761 1.812 1.787 1.636
2.51.403 1.373 1.388 1.237
1.250.988 0.973 0.981 0.830
0.6250.703 0.727 0.715 0.564
0.3120.477 0.497 0.487 0.336
0.1560.322 0.331 0.327 0.176
00.150 0.152 0.151
References
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Function Isoform 1
Subcellular Location Secreted
Protein Families Galanin family
Tissue Specificity Isoform 2 is found in ganglia of ganglioneuroma and ganglioneuroblastoma, as well as in differentiated tumor cells of neuroblastoma tissues. Not found in undifferentiated neuroblasts. Isoform 2 is found in the skin, in pericytes covering microvascular art
Database Links

HGNC: 24840

OMIM: 611178

KEGG: hsa:85569

STRING: 9606.ENSP00000349884

UniGene: Hs.283915

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