Human Galanin-like peptide(GALP) ELISA kit

Code CSB-EL009222HU
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
galanin-like peptide
Alternative Names
GALP ELISA Kit; Galanin-like peptide ELISA Kit
Abbreviation
GALP
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %87-97
1:2Average %101
Range %97-103
1:4Average %95
Range %88-98
1:8Average %99
Range %94-102
Recovery
The recovery of human GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-102
EDTA plasma (n=4)9488-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.057 2.080 2.069 1.918
51.761 1.812 1.787 1.636
2.51.403 1.373 1.388 1.237
1.250.988 0.973 0.981 0.830
0.6250.703 0.727 0.715 0.564
0.3120.477 0.497 0.487 0.336
0.1560.322 0.331 0.327 0.176
00.150 0.152 0.151
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human GALP ELISA Kit was designed for the quantitative measurement of Human GALP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

Function
(From Uniprot)
Hypothalamic neuropeptide which binds to the G-protein-coupled galanin receptors (GALR1, GALR2 and GALR3). Involved in a large number of putative physiological functions in CNS homeostatic processes, including the regulation of gonadotropin-releasing hormone secretion.; Exhibits potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, a physiologic effects which does not appear to be mediated via GALR1 or GALR2. Exhibits antimicrobial activity against Gram-negative bacterias, inducing bacterial membrane blebbing.
Gene References into Functions
  1. results indicated that obese individuals have higher plasma GAL and GALP concentrations and both peptide concentrations were positively correlative to triglyceride concentrations in obese human PMID: 27474259
  2. One isoform (alarin) of this gene but not the other (galanin-like peptide) displays antimicrobial activity against E. coli. PMID: 23537644
  3. This review presents considerable evidence that has accumulated implicating both galanin and GALP as playing important roles in regulating food and water intake behavior and related neuroendocrine functions. PMID: 16787232
  4. describes cloning of the full-length human GALP PMID: 10601261
  5. describes the gene structure of human GALP. PMID: 11861493
  6. reports immunostaining of human GALP in the dermal microvasculature. PMID: 17535903

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Subcellular Location
Secreted.
Protein Families
Galanin family
Tissue Specificity
Isoform 2 is found in ganglia of ganglioneuroma and ganglioneuroblastoma, as well as in differentiated tumor cells of neuroblastoma tissues. Not found in undifferentiated neuroblasts. Isoform 2 is found in the skin, in pericytes covering microvascular art
Database Links

HGNC: 24840

OMIM: 611178

KEGG: hsa:85569

STRING: 9606.ENSP00000349884

UniGene: Hs.283915

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