Human Glypican-4(GPC4) ELISA kit

Human Glypican-4(GPC4) ELISA kit

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Human Glypican-4(GPC4) ELISA kit

Name: Human Glypican-4(GPC4) ELISA kit
Brand: CUSABIO
SKU: CSB-EL009706HU

^Instructions

Code	CSB-EL009706HU
Size	96T,5×96T,10×96T
Trial Size
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Target Background

Target Name

glypican 4

Alternative Names

dJ900E8.1 glypican 4 ELISA Kit; Glypican proteoglycan 4 ELISA Kit; Gpc4 ELISA Kit; GPC4_HUMAN ELISA Kit; K Glypican ELISA Kit; K-glypican ELISA Kit; Kglypican ELISA Kit; OTTHUMP00000024052 ELISA Kit; Secreted glypican 4 ELISA Kit; Secreted glypican-4 ELISA Kit; UNQ474/PRO937 ELISA Kit

Abbreviation

GPC4

Uniprot No.

O75487

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GPC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-104
1:2	Average %	92
	Range %	88-96
1:4	Average %	87
	Range %	81-94
1:8	Average %	98
	Range %	92-106

Recovery

The recovery of human GPC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	95	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.254	2.355	2.305	2.163
20	1.428	1.528	1.478	1.336
10	0.935	0.956	0.946	0.804
5	0.556	0.567	0.562	0.420
2.5	0.372	0.361	0.367	0.225
1.25	0.268	0.257	0.263	0.121
0.625	0.215	0.212	0.214	0.072
0	0.142	0.141	0.142

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

7-14 working days

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Function (From Uniprot)	Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity).
Gene References into Functions	The T allele of GPC4 may represent a risk factor for Epstein-Barr virus-associated gastric carcinoma. PMID: 27071854 According to behavioral studies, downregulation of Gpc4 by Gpc4 siRNA decreased spontaneous seizure frequency, while upregulation of Gpc4 by recombinant Gpc4 overexpression led to a converse result. These findings support the hypothesis that increased expression of Gpc4 in the brain is associated with epileptic seizures. PMID: 27425250 serum level elevated in polycystic ovary syndrome and correlated with fat distribution and cardiovascular risk PMID: 26486309 Data suggest gender difference in circulating GPC4 levels in nonalcoholic fatty liver disease; GPC4 levels in women appear to correlate with cardiometabolic risk factors (adiposity/body fat distribution, insulin resistance, and arterial stiffness). PMID: 23633195 new function of miR-125a by targeting gene glypican-4 in cell growth process PMID: 22644326 Findings establish that Gpc4 acts at the interface of extrinsic and intrinsic signal regulation to fine tune stem cell fate. PMID: 22761013 Show More Hide All
Subcellular Location	Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, SUBCELLULAR LOCATION: Secreted glypican-4: Secreted, extracellular space
Protein Families	Glypican family
Database Links	HGNC: 4452 OMIM: 300168 KEGG: hsa:2239 STRING: 9606.ENSP00000359864 UniGene: Hs.58367

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.