Human Glypican-4(GPC4) ELISA kit

Instructions
Code CSB-EL009706HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name glypican 4
Alternative Names dJ900E8.1 glypican 4 ELISA Kit; Glypican proteoglycan 4 ELISA Kit; Gpc4 ELISA Kit; GPC4_HUMAN ELISA Kit; K Glypican ELISA Kit; K-glypican ELISA Kit; Kglypican ELISA Kit; OTTHUMP00000024052 ELISA Kit; Secreted glypican 4 ELISA Kit; Secreted glypican-4 ELISA Kit; UNQ474/PRO937 ELISA Kit
Abbreviation GPC4
Uniprot No. O75487
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GPC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %92-104
1:2Average %92
Range %88-96
1:4Average %87
Range %81-94
1:8Average %98
Range %92-106
Recovery
The recovery of human GPC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-96
EDTA plasma (n=4)9591-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.254 2.355 2.305 2.163
201.428 1.528 1.478 1.336
100.935 0.956 0.946 0.804
50.556 0.567 0.562 0.420
2.50.372 0.361 0.367 0.225
1.250.268 0.257 0.263 0.121
0.6250.215 0.212 0.214 0.072
00.142 0.141 0.142  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity).
Gene References into Functions
  1. The T allele of GPC4 may represent a risk factor for Epstein-Barr virus-associated gastric carcinoma. PMID: 27071854
  2. According to behavioral studies, downregulation of Gpc4 by Gpc4 siRNA decreased spontaneous seizure frequency, while upregulation of Gpc4 by recombinant Gpc4 overexpression led to a converse result. These findings support the hypothesis that increased expression of Gpc4 in the brain is associated with epileptic seizures. PMID: 27425250
  3. serum level elevated in polycystic ovary syndrome and correlated with fat distribution and cardiovascular risk PMID: 26486309
  4. Data suggest gender difference in circulating GPC4 levels in nonalcoholic fatty liver disease; GPC4 levels in women appear to correlate with cardiometabolic risk factors (adiposity/body fat distribution, insulin resistance, and arterial stiffness). PMID: 23633195
  5. new function of miR-125a by targeting gene glypican-4 in cell growth process PMID: 22644326
  6. Findings establish that Gpc4 acts at the interface of extrinsic and intrinsic signal regulation to fine tune stem cell fate. PMID: 22761013

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, SUBCELLULAR LOCATION: Secreted glypican-4: Secreted, extracellular space
Protein Families Glypican family
Database Links

HGNC: 4452

OMIM: 300168

KEGG: hsa:2239

STRING: 9606.ENSP00000359864

UniGene: Hs.58367

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