GPC4 Antibody

Code CSB-PA009706LA01HU
Size US$166
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  • Western blot
    All lanes: GPC4 antibody at 8µg/ml + Mouse brain tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 63, 55 kDa
    Observed band size: 63 kDa

  • Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA009706LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA009706LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA009706LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) GPC4 Polyclonal antibody
Uniprot No.
Target Names
GPC4
Alternative Names
dJ900E8.1 glypican 4 antibody; Glypican proteoglycan 4 antibody; Gpc4 antibody; GPC4_HUMAN antibody; K Glypican antibody; K-glypican antibody; Kglypican antibody; OTTHUMP00000024052 antibody; Secreted glypican 4 antibody; Secreted glypican-4 antibody; UNQ474/PRO937 antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Glypican-4 protein (19-210AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The GPC4 Antibody (Product code: CSB-PA009706LA01HU) is Non-conjugated. For GPC4 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA009706LB01HU GPC4 Antibody, HRP conjugated ELISA
FITC CSB-PA009706LC01HU GPC4 Antibody, FITC conjugated
Biotin CSB-PA009706LD01HU GPC4 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

In the production of the GPC4 polyclonal antibody, a rabbit was initially immunized with the recombinant human Glypican-4 protein (19-210aa). Afterward, the antibodies were obtained and extracted from the rabbit serum, and subsequently purified using protein G affinity chromatography.

The resulting GPC4 polyclonal antibody possesses a purity level exceeding 95%, indicating that it contains almost no impurities. It has been demonstrated to recognize and bind to GPC4 protein in human and mouse samples. In addition, the versatility of the GPC4 polyclonal antibody has been demonstrated through its successful validation in various applications, such as ELISA, WB, IHC, and IF, indicating its effectiveness in a range of experimental settings.

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Target Background

Function
Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.
Gene References into Functions
  1. The T allele of GPC4 may represent a risk factor for Epstein-Barr virus-associated gastric carcinoma. PMID: 27071854
  2. According to behavioral studies, downregulation of Gpc4 by Gpc4 siRNA decreased spontaneous seizure frequency, while upregulation of Gpc4 by recombinant Gpc4 overexpression led to a converse result. These findings support the hypothesis that increased expression of Gpc4 in the brain is associated with epileptic seizures. PMID: 27425250
  3. serum level elevated in polycystic ovary syndrome and correlated with fat distribution and cardiovascular risk PMID: 26486309
  4. Data suggest gender difference in circulating GPC4 levels in nonalcoholic fatty liver disease; GPC4 levels in women appear to correlate with cardiometabolic risk factors (adiposity/body fat distribution, insulin resistance, and arterial stiffness). PMID: 23633195
  5. new function of miR-125a by targeting gene glypican-4 in cell growth process PMID: 22644326
  6. Findings establish that Gpc4 acts at the interface of extrinsic and intrinsic signal regulation to fine tune stem cell fate. PMID: 22761013

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.; [Secreted glypican-4]: Secreted, extracellular space.
Protein Families
Glypican family
Database Links

HGNC: 4452

OMIM: 300168

KEGG: hsa:2239

STRING: 9606.ENSP00000359864

UniGene: Hs.58367

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