Human Growth/differentiation factor 5(GDF5) ELISA kit

Code CSB-EL009349HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P43026
Lead Time 5-7 working days
Abbreviation GDF5
Protein Biological Process 1 Developmental Protein
Target Name growth differentiation factor 5
Alias BMP14, CDMP1, LAP4, OS5, SYNS2, OTTHUMP00000030755|cartilage-derived morphogenetic protein-1
Species Homo sapiens (Human)
Protein Biological Process 3 Chondrogenesis
Sample Types serum, plasma, tissue homogenates
Detection Range 28 pg/mL-1800 pg/mL
Sensitivity 7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.
HGNC 4220
MGI 95688
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GDF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 105    
Range % 103-108    
1:2 Average % 93    
Range % 90-97    
1:4 Average % 95    
Range % 87-98    
1:8 Average % 93    
Range % 91-99    
Recovery
The recovery of human GDF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 101 97-105    
EDTA plasma (n=4) 92 88-96    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1800 2.688 2.799 2.744 2.661    
900 2.162 2.287 2.225 2.142    
450 1.374 1.399 1.387 1.304    
225 0.726 0.788 0.757 0.674    
112.5 0.352 0.363 0.358 0.275    
56 0.237 0.244 0.241 0.158    
28 0.144 0.150 0.147 0.064    
0 0.080 0.085 0.083      
    .          
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Function Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction
Involvement in disease Acromesomelic chondrodysplasia, Grebe type (AMDG); Acromesomelic chondrodysplasia, Hunter-Thompson type (AMDH); Brachydactyly C (BDC); Du Pan syndrome (DPS); Symphalangism, proximal 1B (SYM1B); Multiple synostoses syndrome 2 (SYNS2); Brachydactyly A2 (BDA2); Osteoarthritis 5 (OS5); Brachydactyly A1, C (BDA1C)
Subcellular Location Secreted, Cell membrane
Protein Families TGF-beta family
Tissue Specificity Predominantly expressed in long bones during embryonic development. Expressed in monocytes (at protein level).
Database Links

HGNC: 4220

OMIM: 112600

KEGG: hsa:8200

STRING: 9606.ENSP00000363489

UniGene: Hs.1573

Pathway Hippo signaling pathway
TGF-beta signaling pathway

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