Product Details

Target Name

growth differentiation factor 5

Alternative Names

BMP14, CDMP1, LAP4, OS5, SYNS2, OTTHUMP00000030755|cartilage-derived morphogenetic protein-1

Abbreviation

GDF5

Uniprot No.

P43026

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

28 pg/mL-1800 pg/mL

Sensitivity

7 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GDF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	103-108
1:2	Average %	93
	Range %	90-97
1:4	Average %	95
	Range %	87-98
1:8	Average %	93
	Range %	91-99

Recovery

The recovery of human GDF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	97-105
EDTA plasma (n=4)	92	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1800	2.688	2.799	2.744	2.661
900	2.162	2.287	2.225	2.142
450	1.374	1.399	1.387	1.304
225	0.726	0.788	0.757	0.674
112.5	0.352	0.363	0.358	0.275
56	0.237	0.244	0.241	0.158
28	0.144	0.150	0.147	0.064
0	0.080	0.085	0.083
		.

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

5-7 working days

Still Have Questions?

Leave a Message or Start an on-line Chat

Target Data

Function	Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction
Involvement in disease	Acromesomelic chondrodysplasia, Grebe type (AMDG); Acromesomelic chondrodysplasia, Hunter-Thompson type (AMDH); Brachydactyly C (BDC); Du Pan syndrome (DPS); Symphalangism, proximal 1B (SYM1B); Multiple synostoses syndrome 2 (SYNS2); Brachydactyly A2 (BDA2); Osteoarthritis 5 (OS5); Brachydactyly A1, C (BDA1C)
Subcellular Location	Secreted, Cell membrane
Protein Families	TGF-beta family
Tissue Specificity	Predominantly expressed in long bones during embryonic development. Expressed in monocytes (at protein level).
Database Links	HGNC: 4220 OMIM: 112600 KEGG: hsa:8200 STRING: 9606.ENSP00000363489 UniGene: Hs.1573

CloseMessage

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How did you know this promotion?

Email

Distributor

Search engine

Advertisement

Others
Check Code：

Code	CSB-EL009349HU
Size	96T,5×96T,10×96T
See More Details

Human Growth/differentiation factor 5(GDF5) ELISA kit

Product Details

Related Products

Target Data

Most popular with customers

CloseMessage