Human Growth/differentiation factor 5(GDF5) ELISA kit

Instructions
Code CSB-EL009349HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name growth differentiation factor 5
Alternative Names BMP14 ELISA Kit; Cartilage derived morphogenetic protein 1 ELISA Kit; Cartilage-derived morphogenetic protein 1 ELISA Kit; CDMP-1 ELISA Kit; CDMP1 ELISA Kit; GDF-5 ELISA Kit; Gdf5 ELISA Kit; GDF5_HUMAN ELISA Kit; Growth differentiation factor 5 ELISA Kit; Growth/differentiation factor 5 ELISA Kit; LAP4 ELISA Kit; OS5 ELISA Kit; Radotermin ELISA Kit; SYNS2 ELISA Kit
Abbreviation GDF5
Uniprot No. P43026
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 28 pg/mL-1800 pg/mL
Sensitivity 7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GDF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 105    
Range % 103-108    
1:2 Average % 93    
Range % 90-97    
1:4 Average % 95    
Range % 87-98    
1:8 Average % 93    
Range % 91-99    
Recovery
The recovery of human GDF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 101 97-105    
EDTA plasma (n=4) 92 88-96    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1800 2.688 2.799 2.744 2.661    
900 2.162 2.287 2.225 2.142    
450 1.374 1.399 1.387 1.304    
225 0.726 0.788 0.757 0.674    
112.5 0.352 0.363 0.358 0.275    
56 0.237 0.244 0.241 0.158    
28 0.144 0.150 0.147 0.064    
0 0.080 0.085 0.083      
    .          
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction. Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG. Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
Gene References into Functions
  1. We found a strong association between the TT genotype and the risk of developing Knee Osteoarthritis (OR = 1.7, 95% CI = 1.12-2.8, p = 0.014), but not in the heterozygous TC state (OR = 1.56, CI 95% = 0.58-4.17, p = 0.367). PMID: 30044130
  2. study shows that there exists a relationship between GDF5 (SNP rs143383) and Developmental dysplasia of the hip (DDH) in our population. Second, we found for the first time that the genotype TT and the T allele were overly expressed in the patients and the fathers. More studies on the confirmation of this genetic marker for DDH are called for. PMID: 29797005
  3. Two Pakistani families with sequence variants in GDF5 and TRPS1 causing brachydactyly type C and tricho-rhino-phalangeal syndrome type III are described. PMID: 29436063
  4. The dysfunctional gene GDF5 was successfully corrected in adipose tissue-derived mesenchymal stem cells using a pair of transcription activatorlike effector nucleases. PMID: 29393424
  5. No association has been found between GDF5 +104 T/C promoter polymorphism and osteoarthritis in the Eastern Turkey population. PMID: 28886316
  6. The results of the current study revealed that SNP rs143383 of GDF5 is a compelling risk factor for knee OA [Osteoarthritis] and that GDF5 has an etiological effect on the development of OA [Osteoarthritis]. PMID: 29056119
  7. A Study of IL-1beta, MMP-3, TGF-beta1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population PMID: 28676856
  8. BMP-14 rs143383 polymorphism reduced the susceptibility to knee osteoarthritis (OA) and hand OA not only in total analysis but also in subgroup analysis; BMP-14 rs143383 polymorphism may be a protective factor against OA occurrence PMID: 29049177
  9. The structure of Grem2-GDF5 complex has revealed a number of key findings for DAN-family mediated BMP2 inhibition. PMID: 27524626
  10. miR-615-3p negatively regulates the osteogenic differentiation of hLF cells through post-transcriptionally suppressing osteogenic regulators GDF5 and FOXO1. PMID: 28460412
  11. p38, c-jun, and NFkappaB pathways activated during intervertebral disc degeneration by IL-1beta but not GDF-5 PMID: 27391542
  12. GDF5 elicited significant (p < 0.05) changes in the expression of anabolic, catabolic and hypertrophic genes with several consistent effects in healthy donors and in OA patients PMID: 28481944
  13. GDF5 was up regulated in patients after chronic rhinosinusitis developing osteitis. PMID: 27888647
  14. Titanium (Ti) surface modification with the combination of hBMP-2 and hGDF-5 for the two growth factor-coated Ti implants can improve the clinical properties of implants for orthopedic and dental applications. PMID: 28124978
  15. he large array of modular enhancers for Gdf5 provide a new foundation for studying the spatial specificity of joint patterning in vertebrates, as well as new candidates for regulatory regions that may also influence osteoarthritis risk in human population PMID: 27902701
  16. The purpose of this study is to investigate the immunohistochemical expression of cytokeratin 18 (CK18) and the reactivity to GDF5 (CDMP-1), called the morphogenetic protein-1, cartilage-derived, in lingual squamous cell carcinoma. PMID: 27151703
  17. homozygous sequence variants in the GDF5 gene underlie acromesomelic dysplasia type-grebe in consanguineous families PMID: 27577507
  18. The prevention of IL-1Beta-induced nucleus pulposus extracellular matrix degeneration by miR-7 silencing was attenuated by GDF5 siRNA. PMID: 27583982
  19. Mutations in three genes (GDF5, NPR2, BMPR1B) have been reported to cause different forms of acromesomelic dysplasia PMID: 26926249
  20. we demonstrate that the transforming growth factor-beta1 and the growth differentiation factor 5 synergistically drive the nucleopulpogenic differentiation process. The commitment of the hASCs was robust and highly specific as attested by the expression of NP-related genes characteristic of young healthy human NP cells PMID: 26661057
  21. The data suggest that Ad-GDF-5 gene therapy is a potential treatment for IDD, which restores the functions of degenerative intervertebral disc through enhancing the ECM production of human NP cells. PMID: 26739524
  22. An association of SNP in GDF5 with temporomandibular joint osteoarthritis in female Han Chinese. PMID: 25757091
  23. results demonstrate that SNP rs143383 of GDF5 is a compelling risk factor for both knee and hand osteoarthritis (OA) and provide further support for GDF5 in the etiology of OA [meta-analysis] PMID: 25894512
  24. Two novel homozygous missense mutations in the GDF5 gene cause brachydactyly type C. PMID: 25820810
  25. our results showed that GDF-5 and BMPRII expressed both in normal and degenerated intervertebral disc tissues, and GDF-5 might have an inhibition effect on degenerated lumbar intervertebral discs PMID: 25755766
  26. This meta-analysis finds that the C allele and CC genotype of the GDF5 gene are protective for knee osteoarthritis susceptibility. PMID: 25467786
  27. Our results revealed that the GDF5 SNP was associated with susceptibility to the meniscus injury and postoperative function recovery in Chinese male soldiers. PMID: 24227118
  28. missense mutations p.T201P and p.L263P interfere with the protein structure and thereby reduce the amount of fully processed, biologically active GDF5, finally causing the clinical loss of function phenotype. PMID: 25092592
  29. The proregion is stabilized by an intramolecular disulfide bond. The isolated proregion folds independently of the mature domain. PMID: 25174448
  30. Growth differentiation factor 5 and canonical Wnt signaling may contribute to molecular mechanisms of osteoarthritis. PMID: 24561281
  31. These results suggest that obesity leads to upregulation of GDF5 expression responsible for the promotion of brown adipogenesis through a mechanism relevant to activation of the NF-kappaB pathway. PMID: 25223801
  32. results suggested that GDF5 polymorphism is associated with susceptibility to symptomatic lumbar disc herniation in Chinese Han population and type II collagen in the nucleus pulposus may be a factor in susceptibility to symptomatic lumbar disc herniation PMID: 24105021
  33. Osteoarthritis chondrocytes do not respond in a predictable manner to culture with exogenous GDF5. PMID: 24466161
  34. High GDF5 expression is associated with osteoarthritis. PMID: 24861163
  35. The expression of growth differentiation factor 5 (GDF5) and aggrecan in 15 cases of salivary gland pleomorphic adenomas, was investigated. PMID: 24398992
  36. established an association between two SNPs (rs224332 and rs224333) of GDF5 and DDH development in a female Chinese population. PMID: 24114442
  37. In vitro findings suggest that the degenerating disc milieu, with high proinflammatory cytokine levels, may limit expression of GDF-5, resulting in limited regenerative capacity of the intact disc. PMID: 24582800
  38. These novel insights into the biology of GDF5 might also provide further clues on the pathophysiology of OA. PMID: 24098149
  39. The novel missense mutation p.Leu176Pro causes impaired secretion of GDF5 in Brachydactyly type C and mild Grebe type chondrodyslplasia. PMID: 23812741
  40. GDF5 is the only osteoarthritis susceptibility gene so far identified with definite evidence.[Review] PMID: 24003854
  41. Overall, a statistically significant association was found between the +104T/C polymorphism of GDF5 and risk of knee osteoarthritis PMID: 23151597
  42. GDF5 harbors a C/A transversion located -41 bp relative to the transcription start site that leads to increased gene expression. PMID: 22929025
  43. GDF5 polymorphisms are associated with susceptibility to low back pain during military training in Chinese soldiers. PMID: 23725396
  44. In conclusion, the rs143383 variant was not found to associate with the risk of ACL rupture. PMID: 23090674
  45. we have identified four trans-acting factors that are binding to GDF5, three of which are modulating GDF5 expression via the OA susceptibility locus rs143383. PMID: 23825960
  46. Although the effect size of the association between OA and GDF5 is small, there is suggestive evidence for an association. PMID: 23423687
  47. GDF5 regulates TGF-beta-dependent angiogenesis in breast carcinoma cells. PMID: 23226264
  48. Growth differentiation factor 5 modulation of chondrogenesis of self-assembled constructs involves gap junction-mediated intercellular communication. PMID: 23121099
  49. analysis of positive selection on the osteoarthritis-risk and decreased-height associated variants at the GDF5 gene in East Asians PMID: 22905146
  50. Our findings in 5 population cohorts from Northern Europe indicate that a variant in the GDF5 gene is a risk factor for lumbar disc degeneration in women. PMID: 21360499

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Involvement in disease Acromesomelic chondrodysplasia, Grebe type (AMDG); Acromesomelic chondrodysplasia, Hunter-Thompson type (AMDH); Brachydactyly C (BDC); Du Pan syndrome (DPS); Symphalangism, proximal 1B (SYM1B); Multiple synostoses syndrome 2 (SYNS2); Brachydactyly A2 (BDA2); Osteoarthritis 5 (OS5); Brachydactyly A1, C (BDA1C)
Subcellular Location Secreted. Cell membrane.
Protein Families TGF-beta family
Tissue Specificity Predominantly expressed in long bones during embryonic development. Expressed in monocytes (at protein level).
Database Links

HGNC: 4220

OMIM: 112600

KEGG: hsa:8200

STRING: 9606.ENSP00000363489

UniGene: Hs.1573

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