Recombinant Human Growth/differentiation factor 5(GDF5)

Code CSB-AP005981HU
Size Pls inquire other sizes
Source E.Coli
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Product Details

Purity > 95 % as determined by SDS-PAGE and HPLC analyses.
Endotoxin Less than 0.1 EU/ug as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
Target Names GDF5
Uniprot No. P43026
Research Area Signal Transduction
Alternative Names BMP14; Cartilage derived morphogenetic protein 1; Cartilage-derived morphogenetic protein 1; CDMP-1; CDMP1; GDF-5; Gdf5; GDF5_HUMAN; Growth differentiation factor 5; Growth/differentiation factor 5; LAP4; OS5; Radotermin; SYNS2
Species Homo sapiens (Human)
Expression Region 382-501aa
Complete Sequence APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
Mol. Weight 13.6 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction
Gene References into Functions
  1. We found a strong association between the TT genotype and the risk of developing Knee Osteoarthritis (OR = 1.7, 95% CI = 1.12-2.8, p = 0.014), but not in the heterozygous TC state (OR = 1.56, CI 95% = 0.58-4.17, p = 0.367). PMID: 30044130
  2. study shows that there exists a relationship between GDF5 (SNP rs143383) and Developmental dysplasia of the hip (DDH) in our population. Second, we found for the first time that the genotype TT and the T allele were overly expressed in the patients and the fathers. More studies on the confirmation of this genetic marker for DDH are called for. PMID: 29797005
  3. Two Pakistani families with sequence variants in GDF5 and TRPS1 causing brachydactyly type C and tricho-rhino-phalangeal syndrome type III are described. PMID: 29436063
  4. The dysfunctional gene GDF5 was successfully corrected in adipose tissue-derived mesenchymal stem cells using a pair of transcription activatorlike effector nucleases. PMID: 29393424
  5. No association has been found between GDF5 +104 T/C promoter polymorphism and osteoarthritis in the Eastern Turkey population. PMID: 28886316
  6. The results of the current study revealed that SNP rs143383 of GDF5 is a compelling risk factor for knee OA [Osteoarthritis] and that GDF5 has an etiological effect on the development of OA [Osteoarthritis]. PMID: 29056119
  7. A Study of IL-1beta, MMP-3, TGF-beta1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population PMID: 28676856
  8. BMP-14 rs143383 polymorphism reduced the susceptibility to knee osteoarthritis (OA) and hand OA not only in total analysis but also in subgroup analysis; BMP-14 rs143383 polymorphism may be a protective factor against OA occurrence PMID: 29049177
  9. The structure of Grem2-GDF5 complex has revealed a number of key findings for DAN-family mediated BMP2 inhibition. PMID: 27524626
  10. miR-615-3p negatively regulates the osteogenic differentiation of hLF cells through post-transcriptionally suppressing osteogenic regulators GDF5 and FOXO1. PMID: 28460412
  11. p38, c-jun, and NFkappaB pathways activated during intervertebral disc degeneration by IL-1beta but not GDF-5 PMID: 27391542
  12. GDF5 elicited significant (p < 0.05) changes in the expression of anabolic, catabolic and hypertrophic genes with several consistent effects in healthy donors and in OA patients PMID: 28481944
  13. GDF5 was up regulated in patients after chronic rhinosinusitis developing osteitis. PMID: 27888647
  14. Titanium (Ti) surface modification with the combination of hBMP-2 and hGDF-5 for the two growth factor-coated Ti implants can improve the clinical properties of implants for orthopedic and dental applications. PMID: 28124978
  15. he large array of modular enhancers for Gdf5 provide a new foundation for studying the spatial specificity of joint patterning in vertebrates, as well as new candidates for regulatory regions that may also influence osteoarthritis risk in human population PMID: 27902701
  16. The purpose of this study is to investigate the immunohistochemical expression of cytokeratin 18 (CK18) and the reactivity to GDF5 (CDMP-1), called the morphogenetic protein-1, cartilage-derived, in lingual squamous cell carcinoma. PMID: 27151703
  17. homozygous sequence variants in the GDF5 gene underlie acromesomelic dysplasia type-grebe in consanguineous families PMID: 27577507
  18. The prevention of IL-1Beta-induced nucleus pulposus extracellular matrix degeneration by miR-7 silencing was attenuated by GDF5 siRNA. PMID: 27583982
  19. Mutations in three genes (GDF5, NPR2, BMPR1B) have been reported to cause different forms of acromesomelic dysplasia PMID: 26926249
  20. we demonstrate that the transforming growth factor-beta1 and the growth differentiation factor 5 synergistically drive the nucleopulpogenic differentiation process. The commitment of the hASCs was robust and highly specific as attested by the expression of NP-related genes characteristic of young healthy human NP cells PMID: 26661057
  21. The data suggest that Ad-GDF-5 gene therapy is a potential treatment for IDD, which restores the functions of degenerative intervertebral disc through enhancing the ECM production of human NP cells. PMID: 26739524
  22. An association of SNP in GDF5 with temporomandibular joint osteoarthritis in female Han Chinese. PMID: 25757091
  23. results demonstrate that SNP rs143383 of GDF5 is a compelling risk factor for both knee and hand osteoarthritis (OA) and provide further support for GDF5 in the etiology of OA [meta-analysis] PMID: 25894512
  24. Two novel homozygous missense mutations in the GDF5 gene cause brachydactyly type C. PMID: 25820810
  25. our results showed that GDF-5 and BMPRII expressed both in normal and degenerated intervertebral disc tissues, and GDF-5 might have an inhibition effect on degenerated lumbar intervertebral discs PMID: 25755766
  26. This meta-analysis finds that the C allele and CC genotype of the GDF5 gene are protective for knee osteoarthritis susceptibility. PMID: 25467786
  27. Our results revealed that the GDF5 SNP was associated with susceptibility to the meniscus injury and postoperative function recovery in Chinese male soldiers. PMID: 24227118
  28. missense mutations p.T201P and p.L263P interfere with the protein structure and thereby reduce the amount of fully processed, biologically active GDF5, finally causing the clinical loss of function phenotype. PMID: 25092592
  29. The proregion is stabilized by an intramolecular disulfide bond. The isolated proregion folds independently of the mature domain. PMID: 25174448
  30. Growth differentiation factor 5 and canonical Wnt signaling may contribute to molecular mechanisms of osteoarthritis. PMID: 24561281
  31. These results suggest that obesity leads to upregulation of GDF5 expression responsible for the promotion of brown adipogenesis through a mechanism relevant to activation of the NF-kappaB pathway. PMID: 25223801
  32. results suggested that GDF5 polymorphism is associated with susceptibility to symptomatic lumbar disc herniation in Chinese Han population and type II collagen in the nucleus pulposus may be a factor in susceptibility to symptomatic lumbar disc herniation PMID: 24105021
  33. Osteoarthritis chondrocytes do not respond in a predictable manner to culture with exogenous GDF5. PMID: 24466161
  34. High GDF5 expression is associated with osteoarthritis. PMID: 24861163
  35. The expression of growth differentiation factor 5 (GDF5) and aggrecan in 15 cases of salivary gland pleomorphic adenomas, was investigated. PMID: 24398992
  36. established an association between two SNPs (rs224332 and rs224333) of GDF5 and DDH development in a female Chinese population. PMID: 24114442
  37. In vitro findings suggest that the degenerating disc milieu, with high proinflammatory cytokine levels, may limit expression of GDF-5, resulting in limited regenerative capacity of the intact disc. PMID: 24582800
  38. These novel insights into the biology of GDF5 might also provide further clues on the pathophysiology of OA. PMID: 24098149
  39. The novel missense mutation p.Leu176Pro causes impaired secretion of GDF5 in Brachydactyly type C and mild Grebe type chondrodyslplasia. PMID: 23812741
  40. GDF5 is the only osteoarthritis susceptibility gene so far identified with definite evidence.[Review] PMID: 24003854
  41. Overall, a statistically significant association was found between the +104T/C polymorphism of GDF5 and risk of knee osteoarthritis PMID: 23151597
  42. GDF5 harbors a C/A transversion located -41 bp relative to the transcription start site that leads to increased gene expression. PMID: 22929025
  43. GDF5 polymorphisms are associated with susceptibility to low back pain during military training in Chinese soldiers. PMID: 23725396
  44. In conclusion, the rs143383 variant was not found to associate with the risk of ACL rupture. PMID: 23090674
  45. we have identified four trans-acting factors that are binding to GDF5, three of which are modulating GDF5 expression via the OA susceptibility locus rs143383. PMID: 23825960
  46. Although the effect size of the association between OA and GDF5 is small, there is suggestive evidence for an association. PMID: 23423687
  47. GDF5 regulates TGF-beta-dependent angiogenesis in breast carcinoma cells. PMID: 23226264
  48. Growth differentiation factor 5 modulation of chondrogenesis of self-assembled constructs involves gap junction-mediated intercellular communication. PMID: 23121099
  49. analysis of positive selection on the osteoarthritis-risk and decreased-height associated variants at the GDF5 gene in East Asians PMID: 22905146
  50. Our findings in 5 population cohorts from Northern Europe indicate that a variant in the GDF5 gene is a risk factor for lumbar disc degeneration in women. PMID: 21360499
  51. Crystals of GDF5 and BMP receptor IA complex belonged to a monoclinic space group: either I2, with unit-cell parameters a = 63.81, b = 62.85, c = 124.99 A, beta = 95.9 degrees , or C2, with unit-cell parameters a = 132.17, b = 62.78, c = 63.53 A, beta = 112.8 degrees PMID: 21543859
  52. In conclusion, we find that GDF5, but not COG5 or MCF2L, influence the extent of radiographic damage in knee osteoarthritis PMID: 22615457
  53. A novel molecular mechanism of a GDF5 mutation affecting chondrogenesis and osteogenesis, is reported. PMID: 21976273
  54. [review] In a case of identical female twins in two unrelated families, CDMP-1 mutation is not associated with dysplastic developmental spondylolisthesis; however, mutant CDMP-1 may be an etiology for at least a subpopulation of patients. PMID: 21270694
  55. GDF5 polymorphism has an association with knee osteoarthritis in Thai ethnic. PMID: 21936909
  56. Data demonstrates that the genetic effect of the rs143383 SNP on GDF5 expression is modulated epigenetically by DNA methylation. PMID: 21642387
  57. human articular chondrocyte dedifferentiation is associated with alterations in expression patterns of GDF-5 and its receptors PMID: 19874419
  58. T allele of GDF5 rs143833 is associated with a 17% increased risk of knee osteoarthritis & when all data were analysed the genetic association reached genome-wide significance with p=6.2x10-11 in all samples and with p=8.3x10-9 in European samples PMID: 20870806
  59. BDA1 (semidominant brachydactyly A1) can result from semidominant mutations in GDF5. PMID: 20683927
  60. The mutant GDF-5 showed superior bone formation capacity than GDF-5. PMID: 20170953
  61. study describes that specific mutations of a single amino acid in GDF5 cause congenital fusion of joints; show that the mutant GDF5 is no longer inhibited by Noggin PMID: 19956691
  62. the inhibition of apoptosis by BMP2 and GDF5 does not depend on more complex signal transduction pathways such as smad and MAPK signaling but on direct stabilization of XIAP by BMPR2. PMID: 19782107
  63. CDMP may be a useful growth factor to stimulate proteoglycan production in the human degenerate intervertebral disc PMID: 19754961
  64. A missense mutation in the mature domain of CDMP1 found in a kindred affected with fibular hypoplasia and complex brachydactyly likely causes a CDMP1 conformational change that influences expression of genes required for normal bone develoment. PMID: 12121354
  65. CDMP1 plays an important role in the regulation of axial bone growth during development but its absence does not impair other developmental processes. PMID: 12124730
  66. brachydactyly type C results from functional haploinsufficiency for GDF5 PMID: 12357473
  67. pleomorphic adenoma expressed cartilage derived morphogenetic protein 1 but not 2 PMID: 12707774
  68. CDMP1 responsible for Brachydactyly type C PMID: 14735582
  69. Role of GDF5 in signal pathway. PMID: 15569154
  70. GDF5 is a novel brachydactyly type A2 causing gene. PMID: 16014698
  71. Results describe 2 mutations in growth and differentiation factor 5 (GDF5) that alter receptor-binding affinities. PMID: 16127465
  72. promotes proliferation and differentiation of calvarial cells PMID: 16499717
  73. Presence of only one monomer in the asymmetric unit, resulting in a high solvent content of 72% in the crystal. PMID: 16508114
  74. GDF5 could be involved in the process of angiogenesis. GDF5 signal transduction pathway PMID: 16716349
  75. GDF5 is a susceptibility gene for osteoarthritis and decreased GDF5 expression is involved in the pathogenesis of osteoarthritis. PMID: 17384641
  76. Successful treatment with infliximab of refractory rheumatoid arthritis in a male with 'GDF5 brachydaltyly' is reported. PMID: 17602228
  77. small but persistent imbalance of GDF5 expression throughout life therefore appears to render an individual more susceptible to osteoarthritis PMID: 17616513
  78. the +104T/C; rs143383 GDF5 core promoter polymorphism is not a risk factor for osteoarthritis etiology in Greek Caucasians PMID: 17676627
  79. A GDF5/bone morphogenetic protein 4 (BMP4)selective binding protein exists in human pulmonary artery smooth muscle cells. PMID: 17989347
  80. observations indicate that GDF-5 stimulates osteogenic differentiation and has a potential to induce angiogenesis through osteoblast-derived VEGF-A in bone. PMID: 18075819
  81. Common variants in the GDF5-UQCC region are associated with variation in human height. PMID: 18193045
  82. Unprocessed proGDF5 is virtually inactive but can be proteolytically activated by different enzymes such as trypsin, furin, and MMP3. PMID: 18203755
  83. Novel point mutations in GDF5 associated with two distinct limb malformations in Chinese: brachydactyly type C and proximal symphalangism. PMID: 18283415
  84. The results showed a positive association of the GDF5 single-nucleotide polymorphism with knee osteoarthritis for Europeans as well as for Asians. PMID: 18299287
  85. A patient with Du Pan type chondrodysplasia has two novel mutations and compound heterozygosity for GDF5. PMID: 18629880
  86. present work shows that the functional GDF5 polymorphism associated with osteoarthritis risk demonstrates a similar role in rheumatoid arthritis susceptibility PMID: 18697781
  87. expressed in synovial fibroblasts and may counteract macrophage infiltration PMID: 18830904
  88. gene growth differentiate factor 5 is important in the aetiology of congenital dysplasia of the hip. PMID: 18947434
  89. Results describe a novel missense mutation in the gene for growth differentiation factor 5 that results in a phenotype similar to Grebe-type chondrodysplasia. PMID: 18979166
  90. BMP-2 and BMP-14 expression was strongest in areas of cartilage formation and, to a lesser extent, in areas of bone formation. PMID: 19023570
  91. Results show that GDF5 gene variants are associated with hand, knee osteoarthritis and fracture risk in elderly women and replicates previous association between GDF5 variation and height. PMID: 19029166
  92. The study presented here confirms that CDMP1 plays an important role in the regulation of the development skeleton, and its absence does not impair other development processes PMID: 19038017
  93. data confirm that the GDF5 variant is consistently associated with the risk of knee osteoarthritis and ethnic variation in allele frequencies at the DVWA gene was found and no significant association was found in UK or by combining UK and Asian samples. PMID: 19054821
  94. There was a significant reduction in BMP-2 and BMP-14 expression in cartilaginous areas of nonhealing fractures compared to healing fractures PMID: 19058174
  95. Here, we present structural insights into the GDF-5:BMPR-IB complex revealing how binding specificity for BMPR-IB is generated on a molecular level. PMID: 19229295
  96. The cystine knot of homodimeric recombinant GDF5 exhibits the pattern Cys1-Cys5, Cys2-Cys6, and Cys3-Cys7 (three intrachain disulfide bonds), and the monomers are connected by a single interchain disulfide bridge (Cys4-Cys4). PMID: 19393216
  97. Evidence of an association between the GDF5 rs143383 polymorphism and osteoarthritis (OA) is substantially strong, but the genetic effects are consistent across different populations only for knee OA. PMID: 19479880
  98. OA susceptibility mediated by polymorphism in GDF5 is not restricted to cartilage, emphasizing the need to consider the disease as involving the whole joint. PMID: 19565498
  99. crystals consisted of the full ternary complex GDF5-BRIB-ActRIIB, but only diffracted to low resolution PMID: 19652338

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Involvement in disease Acromesomelic chondrodysplasia, Grebe type (AMDG); Acromesomelic chondrodysplasia, Hunter-Thompson type (AMDH); Brachydactyly C (BDC); Du Pan syndrome (DPS); Symphalangism, proximal 1B (SYM1B); Multiple synostoses syndrome 2 (SYNS2); Brachydactyly A2 (BDA2); Osteoarthritis 5 (OS5); Brachydactyly A1, C (BDA1C)
Subcellular Location Secreted, Cell membrane
Protein Families TGF-beta family
Tissue Specificity Predominantly expressed in long bones during embryonic development. Expressed in monocytes (at protein level).
Database Links

HGNC: 4220

OMIM: 112600

KEGG: hsa:8200

STRING: 9606.ENSP00000363489

UniGene: Hs.1573

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