Human HERV-K_7p22.1 provirus ancestral Env polyprotein(ERVK6) ELISA kit

Instructions
Code CSB-EL007812HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name endogenous retroviral sequence K, 6
Alternative Names ERVK-6 ELISA Kit; ERVK6Endogenous retrovirus group K member 6 Env polyprotein ELISA Kit; EnvK2 protein ELISA Kit; Envelope polyprotein ELISA Kit; HERV-K(C7) envelope protein ELISA Kit; HERV-K(HML-2.HOM) envelope protein ELISA Kit; HERV-K108 envelope protein ELISA Kit; HERV-K_7p22.1 provirus ancestral Env polyprotein) [Cleaved into: Surface protein ELISA Kit; SU); Transmembrane protein ELISA Kit; TM)] ELISA Kit
Abbreviation ERVK6
Uniprot No. Q69384
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ERVK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 91-99
1:2 Average % 103
Range % 98-106
1:4 Average % 110
Range % 106-114
1:8 Average % 92
Range % 89-97
Recovery
The recovery of human ERVK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 84 81-89
EDTA plasma (n=4) 97 93-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.251 2.301 2.276 2.154
5 1.375 1.368 1.372 1.250
2.5 0.782 0.778 0.780 0.658
1.25 0.503 0.511 0.507 0.385
0.625 0.325 0.336 0.331 0.209
0.312 0.247 0.268 0.258 0.136
0.156 0.192 0.186 0.189 0.067
0 0.121 0.123 0.122  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Citations

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Target Background

Function
(From Uniprot)
Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its original fusogenic properties.
Subcellular Location Transmembrane protein: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Surface protein: Cell membrane, Peripheral membrane protein
Protein Families Beta type-B retroviral envelope protein family, HERV class-II K(HML-2) env subfamily
Tissue Specificity Expressed in testis, and peripheral blood lymphocytes.
Database Links

HGNC: 13915

OMIM: 605626

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