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Human HERV-K_7p22.1 provirus ancestral Env polyprotein(ERVK6) ELISA kit

Citations (6) Protocol MSDS
Code CSB-EL007812HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
endogenous retroviral sequence K, 6
Alternative Names
ERVK-6 ELISA Kit; ERVK6Endogenous retrovirus group K member 6 Env polyprotein ELISA Kit; EnvK2 protein ELISA Kit; Envelope polyprotein ELISA Kit; HERV-K(C7) envelope protein ELISA Kit; HERV-K(HML-2.HOM) envelope protein ELISA Kit; HERV-K108 envelope protein ELISA Kit; HERV-K_7p22.1 provirus ancestral Env polyprotein) [Cleaved into: Surface protein ELISA Kit; SU); Transmembrane protein ELISA Kit; TM)] ELISA Kit
Abbreviation
ERVK6
Uniprot No.
Q69384
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ERVK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 95
Range % 91-99
1:2 Average % 103
Range % 98-106
1:4 Average % 110
Range % 106-114
1:8 Average % 92
Range % 89-97
Recovery
The recovery of human ERVK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 84 81-89
EDTA plasma (n=4) 97 93-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.251 2.301 2.276 2.154
5 1.375 1.368 1.372 1.250
2.5 0.782 0.778 0.780 0.658
1.25 0.503 0.511 0.507 0.385
0.625 0.325 0.336 0.331 0.209
0.312 0.247 0.268 0.258 0.136
0.156 0.192 0.186 0.189 0.067
0 0.121 0.123 0.122 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Human ERVK6 ELISA Kit was designed for the quantitative measurement of Human ERVK6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Citations

Related Products

ERVK-6 Antibodies

ERVK-6 Proteins

Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: No dilution

Review: Very good, very high sensitivity, very high accuracy, very good experimental results, positive data, good fitting curve, the kit is very easy to use, very simple to operate

By Anonymous

Read more reviews >>

Target Background

Function
(From Uniprot)
Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its original fusogenic properties.; SU mediates receptor recognition.; TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane.
Subcellular Location
[Transmembrane protein]: Cell membrane; Single-pass type I membrane protein.; [Surface protein]: Cell membrane; Peripheral membrane protein.; [Endogenous retrovirus group K member 6 Env polyprotein]: Virion.
Protein Families
Beta type-B retroviral envelope protein family, HERV class-II K(HML-2) env subfamily
Tissue Specificity
Expressed in testis, and peripheral blood lymphocytes.
Database Links

HGNC: 13915

OMIM: 605626

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