Human Hepcidin 25(Hepc25)ELISA Kit

Code CSB-E14239h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Abbreviation Hepc25
Species Homo sapiens (Human)
Sample Types
Detection Range
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    

To assess the linearity of the assay, samples were spiked with high concentrations of human Hepc25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 82-94  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 92  
Range % 87-99  
1:8 Average % 95  
Range % 89-100  
The recovery of human Hepc25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-97  
EDTA plasma (n=4) 100 95-102  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
300 2.008 2.128 2.068 1.943  
150 1.497 1.393 1.445 1.320  
75 0.902 0.880 0.891 0.766  
37.5 0.580 0.586 0.583 0.458  
18.75 0.390 0.382 0.386 0.261  
9.38 0.272 0.276 0.274 0.149  
4.69 0.178 0.184 0.181 0.056  
0 0.125 0.124 0.125    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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I am looking for human hepcidin-25 ELISA, which is specific for Bioactive Hep-25 and there is NOT crossreactivity for Hep-20 and Hep-21. I have checked many Hep-25 ELISAs on your website, however could not find any information about the crossreactivity our customer requested. Would you please confirm the crossreactivity about your Hep-25 ELISAs?

Thanks for your inquiry.
The recombinant Hepcidin protein and the synthesized Hep-20, Hep-21 and Hep-22 polypeptides There is no across activity.
Considering that the structure for modification of proteins in native samples is different from recombinant proteins and synthesized polypeptides,
it is not guaranteed that there is no any cross with other isoforms in native samples.Pls let me know if you have any further questions. Thank you.

Re: CSB-E14239h Human Hepcidin 25, Hepc25 ELISA Kit
I am looking for Hepcidin 25 ELISA KIT which does NOT react with “Hepcidin 20” and “Hepcidin 21”.
Can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please send the manual.

Thanks for your inquiry.
This kit is very special and it is based on double antibody sandwich method.
Capture antibody is mouse monoclonal antibody and detection antibody is rat monoclonal antibody.
Standard is produced by a special method.
I will send you the manual by e-mail.
Pls let me know if you have any further questions. Thank you.


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