Human Hydroxymethylglutaryl CoA Reductase (HMG-CoAR) ELISA kit

Instructions
Code CSB-E15964h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Alternative Names LDLCQ3, 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)|hydroxymethylglutaryl-CoA reductase
Abbreviation HMGCR
Uniprot No. P04035
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL- 50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HMG-CoAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %92-100
1:2Average %91
Range %82-96
1:4Average %95
Range %87-100
1:8Average %87
Range %82-98
Recovery
The recovery of HMG-CoAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9691-101
EDTA plasma (n=4)9082-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.325 2.450 2.388 2.271
252.050 2.174 2.112 1.995
12.51.476 1.563 1.520 1.403
6.250.659 0.697 0.678 0.561
3.120.245 0.261 0.253 0.136
1.560.192 0.186 0.189 0.072
0.780.151 0.140 0.146 0.029
00.120 0.113 0.117  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Target Data

Function Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.
Subcellular Location Endoplasmic reticulum membrane, Multi-pass membrane protein
Protein Families HMG-CoA reductase family
Database Links

HGNC: 5006

OMIM: 142910

KEGG: hsa:3156

STRING: 9606.ENSP00000287936

UniGene: Hs.628096

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