Human Hydroxymethylglutaryl CoA Reductase (HMG-CoAR) ELISA kit

Code CSB-E15964h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P04035
Lead Time 3-5 working days
Protein Biological Process 2 Lipogenesis and lipometabolism
Abbreviation HMGCR
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Alias LDLCQ3, 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)|hydroxymethylglutaryl-CoA reductase
Species Homo sapiens (Human)
Protein Biological Process 3 Cholesterol biosynthesis
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL- 50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
HGNC 5006
RGD 2803
MGI 96159
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HMG-CoAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %92-100
1:2Average %91
Range %82-96
1:4Average %95
Range %87-100
1:8Average %87
Range %82-98
Recovery
The recovery of HMG-CoAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9691-101
EDTA plasma (n=4)9082-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.325 2.450 2.388 2.271
252.050 2.174 2.112 1.995
12.51.476 1.563 1.520 1.403
6.250.659 0.697 0.678 0.561
3.120.245 0.261 0.253 0.136
1.560.192 0.186 0.189 0.072
0.780.151 0.140 0.146 0.029
00.120 0.113 0.117  
References
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Function Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.
Subcellular Location Endoplasmic reticulum membrane, Multi-pass membrane protein
Protein Families HMG-CoA reductase family
Database Links

HGNC: 5006

OMIM: 142910

KEGG: hsa:3156

STRING: 9606.ENSP00000287936

UniGene: Hs.628096

Pathway AMPK signaling

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