Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

HMGCR

Uniprot No.

P04035

Research Area

Metabolism

Alternative Names

3 hydroxy 3 methylglutaryl CoA reductase; 3 hydroxy 3 methylglutaryl Coenzyme A reductase; 3 hydroxymethylglutaryl CoA reductase; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH); 3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3H3M; HMDH_HUMAN; HMG CoA reductase; HMG CoAR; HMG-CoA reductase; Hmgcr; Hydroxymethylglutaryl CoA reductase; LDLCQ3; MGC103269; Red

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

588-887aa

Target Protein Sequence

MTRGPVVRLPRACDSAEVKAWLETSEGFAVIKEAFDSTSRFARLQKLHTSIAGRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPAAINWIEGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVKSHMIHNRSKINLQDLQGACTKKT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

36.0kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The production of this recombinant Human HMGCR protein is just like all recombinant proteins. The process involved transfecting E.coli cells with DNA vector containing the template of recombinant DNA. The E.coli cells containing the template were then cultured so that they could transcribe and translate the HMGCR protein. N-terminal 6xHis tag was used in the process. The purity is 90% determined by SDS-PAGE.

HMGCR is a gene providing instructions for making a protein called 3-hydroxy-3-methylglutaryl-coenzyme A reductase (also known as HMG-CoA reductase), which is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol and other isoprenoids. HMG-CoA reductase catalyzes the NADPH-dependent reduction of HMG-CoA to mevalonic acid, a necessary step in the biosynthesis of cholesterol. Currently, this enzyme is the target of the widely available cholesterol-lowering drugs, such as statins, which help treat dyslipidemia.

Target Background

Function

Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis. HMGCR is the main target of statins, a class of cholesterol-lowering drugs.

Gene References into Functions

High HMGR expression is associated with breast cancer metastasis. PMID: 29626418
High HMGCR expression is associated with hypercholesterolemia. PMID: 29678744
The presence of rs17244841 ve rs17238540 mutations in HMGCR causes a significant reduction in total cholesterol and LDL-c levels. PMID: 29096742
A positive relationship emerged between HMG-CoAR, hormone receptors and TAZ/YAP, suggesting a connection between the mevalonate pathway, the hormonal milieu and Hippo in Male breast cancer. Moreover, HMG-CoAR expression may be a favorable prognostic indicator. PMID: 27713571
HMGCR genetic variation is associated with Alzheimer's disease. PMID: 26950278
our study demonstrates A allele of HMGCR rs3846662 acts as a protective factor for late-onset Alzheimer's disease in northern Han Chinese PMID: 27009838
UBXD8 is necessary for sterol-stimulated dislocation of ubiquitylated HMGCR from the endoplasmic reticulum membrane en route to proteasomal degradation, a function dependent on its UBX domain. PMID: 28882874
The docking studies indicate rutin as the best compound that can inhibit HMG-CoA reductase as it had strong binding affinity to the enzyme. PMID: 27216569
Both HMGCR and SQLE promoters have two SREs that may act as a homing region to attract a single SREBP-2 homodimer. PMID: 28342963
To estimate the association between changes in levels of LDL-C (and other lipoproteins) and the risk of cardiovascular events related to variants in the CETP gene, both alone and in combination with variants in the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) gene. PMID: 28846118
Low LDL cholesterol levels due to PCSK9 and HMGCR variants had no causal effect on high risk of Alzheimer's disease, vascular dementia, any dementia, or Parkinson's disease; however, low LDL cholesterol levels may have a causal effect in reducing the risk of Alzheimer's disease. PMID: 28438747
TGF-beta1 induces cholesterol synthesis by increasing HMG-CoA reductase mRNA expression in keratinocytes. PMID: 26932266
Gene expression profile and the biological functions of HMGCR in gastric cancer were studied. It was found that the expression of HMGCR was increased in gastric cancer tissues. Over-expression of HMGCR promoted the growth and migration of gastric cancer cells, while knocking down the expression of HMGCR inhibited the growth, migration and tumorigenesis of gastric cancer cells. PMID: 27085483
In this study, variants in PCSK9 had approximately the same effect as variants in HMGCR on the risk of cardiovascular events and diabetes per unit decrease in the LDL cholesterol level. The effects of these variants were independent and additive. PMID: 27959767
Data show that simvastatin significantly inhibited cellular proliferation, induced cell cycle G1 arrest and apoptosis, and caused cellular stress via reduction in the enzymatic activity of 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGCR). PMID: 26503475
no associations found between HMGCR rs3846662, HMRCR alternative splicing and Alzheimer's disease pathology pathology. PMID: 26541602
results indicate that abnormal lipid metabolism may exist in spontaneous preterm delivery (SPTD) women and the premature fetus and the HMGCR gene may be a susceptible gene for SPTD PMID: 26301579
Report the mechanism of polyphenol binding to and activity regulation of HMGR using molecular docking. PMID: 26357462
the results of the present study showed that luteolin modulates HMGCR transcription by decreasing the expression and nuclear translocation of SREBP-2. PMID: 26302339
Discovery new drug candidates for inhibition of human HMG-CoA reductase through structure-based virtual screening. PMID: 26170618
Results show that HMGCR rs3846662 acts as a potent genetic modifier for Alzheimer's disease risk, age of onset and conversion from mild cognitively impairment PMID: 25023145
study demonstrated the oncogenic roles of HMGCR in glioblastoma cells and HMGCR might be a promising therapeutic target. PMID: 26432005
the 5-aminoimidazole-4-carboxyamide ribonucleoside (AICAR)-induced activation of AMPK directly inhibited the expression of SREBP-2 and HMGCR and HMGCS, and suppressed the TSH-stimulated up-regulation of SREBP-2 in HepG2 cells. PMID: 25933205
Data show that sterols stimulate binding of prenyltransferase UBIAD1 to HMG CoA reductase, which is subject to sterol-accelerated, endoplasmic reticulum (ER)-associated degradation augmented by the nonsterol isoprenoid geranylgeraniol. PMID: 25742604
Human carotid atherosclerotic lesion protein components decrease cholesterol biosynthesis rate in macrophages through 3-hydroxy-3-methylglutaryl-CoA reductase regulation. PMID: 25639207
miR-21 regulates triglyceride and cholesterol metabolism in non-alcoholic fatty liver disease by targeting HMGCR PMID: 25605429
The effect of lower LDL-C on the risk of coronary heart disease mediated by polymorphisms in NPC1L1, HMGCR, or both is approximately the same per unit lower LDL-C and log-linearly proportional to the absolute exposure to lower LDL-C. PMID: 25770315
promoter DNA hypermethylation of the ABCG1 and GALNT2 genes, but not the HMGCR gene, is associated with an increased risk of CHD. PMID: 25084356
Strong HMGCR expression is associated with high response to radiotherapy in ductal carcinoma-in situ. PMID: 24777857
The results show that carriage of SNPs in the HMGCR gene were associated with bodyweight gain and increased risk of type 2 diabetes PMID: 25262344
HMGCR is differentially expressed in colorectal cancer and that positive expression is associated with favourable tumour characteristics and a prolonged survival in unadjusted analysis. PMID: 24708688
HMGCR is upregulated in hepatocellular carcinoma associated with paraneoplastic hypercholesterolemia. PMID: 23549978
findings show that HNRNPA1 modulates the expression level of an alternatively spliced transcript of HMGCR by regulating splicing and altering RNA stability, resulting in reduced HMGCR enzyme activity and increased LDL-Cholesterol uptake PMID: 24001602
Data indicate that RNAi-mediated knockdown of VCP/p97 blocks sterol-accelerated degradation but not ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reduc (HMG CoA reductase) in SV-589 cells. PMID: 24025715
HMGCR-mediated changes in F-actin structure play an important role in the inter-cellular transmission of respiratory syncytial virus infection. PMID: 23994498
Over-expression of HMGCR in esophageal squamous cell carcinoma (ESCC) cells promoted cell growth and migration. PMID: 24390662
knockdown of MARCH6 also controls the level of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase (HMGCR) in hepatocytes and model cell lines PMID: 24449766
Increased cholesterol synthesis mediated by HMGCoA-R under inflammatory stress may be one of the mechanisms for intracellular lipid accumulation and statin resistance. PMID: 24233489
Age and sex modify the contribution of the HMGCR-911 polymorphism to fasting serum total cholesterol, LDL-cholesterol levels and risk of coronary heart disease. PMID: 23933271
These results suggest that dengue virus infection increases intracellular cholesterol levels at early times post infection by triggering the modulation of LDL particles uptake and the increase in the enzymatic activity of HMG-CoA reductase. PMID: 23642566
A novel role for Aup1 in maintenance of intracellular cholesterol homeostasis via mediation of the endoplasmic reticulum associated degradation of HMG-CoA reductase. PMID: 23223569
Statins up-regulate the expression of HMGCR, the major target of autoantibodies in statin-associated immune-mediated necrotizing myopathy. PMID: 21360500
The association of HMGCR promoter region polymorphisms with cholesterol levels and coronary artery disease in patients from Western India are reported. PMID: 22858685
This study was designed to understand the mode of interactions of HMGCR isoform 2 with statins Atorvastatin, Lovastatin, Fluvastatin, Simvastatin, Pravastatin, Rosuvastatin and Cerivastatin. PMID: 22177940
The HMGCR rs3846662 GG genotype was quantitatively documented to be a significant determinant for higher LDL-C level in basal state and possibly in response to atorvastatin. PMID: 21427285
Reductions in plasma insulin may have affected the expression of a key regulatory gene of cholesterol synthesis, HMG-CoA reductase and low-density lipoprotein receptor. PMID: 22024489
HMG-CoA reductase activation and urinary pellet cholesterol elevations in acute kidney injury. PMID: 21799150
HMG-CoA reductase regulation takes place at the levels of transcription, translation, post-translational modification and degradation. (Review) PMID: 21801748
Linalool reduces the expression of 3-hydroxy-3-methylglutaryl CoA reductase via sterol regulatory element binding protein-2- and ubiquitin-dependent mechanisms. PMID: 21944868
inhibition of protein geranylgeranylation markedly attenuated ubiquitylation and dislocation, implicating for the first time a geranylgeranylated protein(s) in the metabolically regulated ERAD of HMGR. PMID: 21778231

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Peroxisome membrane; Multi-pass membrane protein.

Protein Families

HMG-CoA reductase family

Tissue Specificity

[Isoform 1]: Ubiquitously expressed with the highest levels in the cerebellum, fetal brain, testis, skin and adrenal gland.; [Isoform 2]: Detected in the cerebellum, fetal brain, testis and adrenal gland.; [Isoform 3]: Low abundance except in skin, esopha

Database Links

HGNC: 5006

OMIM: 142910

KEGG: hsa:3156

STRING: 9606.ENSP00000287936

UniGene: Hs.628096

Code	CSB-EP010565HU
Abbreviation	Recombinant Human HMGCR protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), partial

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