Human ICOS ligand(ICOSLG) ELISA kit

Instructions
Code CSB-EL010958HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name inducible T-cell co-stimulator ligand
Alternative Names B7 H2 ELISA Kit; B7 homolog 2 ELISA Kit; B7 homologue 2 ELISA Kit; B7 like protein Gl50 ELISA Kit; B7 related protein 1 ELISA Kit; B7-H2 ELISA Kit; B7-like protein Gl50 ELISA Kit; B7-related protein 1 ELISA Kit; B7H2 ELISA Kit; B7RP 1 ELISA Kit; B7RP-1 ELISA Kit; B7RP1 ELISA Kit; CD 275 ELISA Kit; CD275 ELISA Kit; CD275 antigen ELISA Kit; GL 50 ELISA Kit; GL50 ELISA Kit; ICOS L ELISA Kit; ICOS LG ELISA Kit; ICOS ligand ELISA Kit; ICOSL ELISA Kit; ICOSL_HUMAN ELISA Kit; Icoslg ELISA Kit; Inducible T cell co stimulator ligand ELISA Kit; KIAA0653 ELISA Kit; LICOS ELISA Kit; Transmembrane protein B7 H2 ICOS ligand ELISA Kit
Abbreviation ICOSLG
Uniprot No. O75144
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.4 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ICOSLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %82-94
1:2Average %96
Range %89-102
1:4Average %98
Range %95-106
1:5Average %95
Range %91-100
Recovery
The recovery of human ICOSLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9688-103
EDTA plasma (n=4)9490-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
60002.748 2.789 2.769 2.592
30002.375 2.372 2.374 2.197
15001.834 1.816 1.825 1.648
7501.061 1.047 1.054 0.877
3750.632 0.636 0.634 0.457
187.50.427 0.441 0.434 0.257
93.750.259 0.269 0.264 0.087
00.179 0.174 0.177  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity).
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Immunoglobulin superfamily, BTN/MOG family
Tissue Specificity Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leuko
Database Links

HGNC: 17087

OMIM: 605717

KEGG: hsa:102723996

STRING: 9606.ENSP00000384432

UniGene: Hs.14155

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