Human Ig alpha-1 chain C region(IGHA1) ELISA kit

Code CSB-EL011111HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
immunoglobulin heavy constant alpha 1
Alternative Names
IGHA1 ELISA Kit; Immunoglobulin heavy constant alpha 1 ELISA Kit; Ig alpha-1 chain C region ELISA Kit; Ig alpha-1 chain C region BUR ELISA Kit; Ig alpha-1 chain C region TRO ELISA Kit
Abbreviation
IGHA1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
0.49 ng/mL-2000 ng/mL
Sensitivity
0.49 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IGHA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5000 Average % 92
Range % 85-103
1:10000 Average % 91
Range % 85-99
1:20000 Average % 97
Range % 90-108
1:40000 Average % 92
Range % 83-100
Recovery
The recovery of human IGHA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 83-101
EDTA plasma (n=4) 95 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
2000 2.234 2.285 2.260 2.171
500 2.112 2.165 2.139 2.050
125 1.886 1.897 1.892 1.803
31.25 1.265 1.245 1.255 1.166
7.81 0.608 0.587 0.598 0.509
1.95 0.290 0.286 0.288 0.199
0.49 0.136 0.139 0.138 0.049
0 0.086 0.091 0.089  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human IGHA1 ELISA Kit was designed for the quantitative measurement of Human IGHA1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.49 ng/mL-2000 ng/mL and the sensitivity is 0.49 ng/mL.

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Target Background

Function
(From Uniprot)
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. Ig alpha is the major immunoglobulin class in body secretions.
Involvement in disease
A chromosomal aberration involving IGHA1 is found in multiple myeloma (MM) cell lines. Translocation t(1;14)(q21;q32) that forms a FCRL4-IGHA1 fusion protein.
Subcellular Location
Secreted. Cell membrane.
Database Links

HGNC: 5478

OMIM: 146900

UniGene: Hs.699841

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