Human Ig gamma-3 chain C region(IGHG3) ELISA kit

Code CSB-EL011158HU
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
immunoglobulin heavy constant gamma 3 (G3m marker)
Alternative Names
IGHG3Immunoglobulin heavy constant gamma 3 ELISA Kit; HDC ELISA Kit; Heavy chain disease protein ELISA Kit; Ig gamma-3 chain C region ELISA Kit
Abbreviation
IGHG3
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
2.7 ng/mL-2000 ng/mL
Sensitivity
2.7 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IGHG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %99
Range %93-106
1:2000Average %90
Range %85-95
1:4000Average %87
Range %82-91
1:8000Average %93
Range %86-98
Recovery
The recovery of human IGHG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9086-94
EDTA plasma (n=4)9590-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
20000.173 0.172 0.1725
6670.259 0.246 0.2525
2220.367 0.344 0.3555
740.488 0.459 0.4735
250.633 0.624 0.6285
8.20.789 0.855 0.822
2.71.029 1.033 1.031
01.697 1.748 1.7225
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human IGHG3 ELISA Kit was designed for the quantitative measurement of Human IGHG3 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 2.7 ng/mL-2000 ng/mL and the sensitivity is 2.7 ng/mL .

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Target Background

Function
(From Uniprot)
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
Subcellular Location
Secreted. Cell membrane.
Database Links

HGNC: 5527

OMIM: 147120

UniGene: Hs.510635

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