Product Details

Target Name

insulin-like growth factor 2 mRNA binding protein 2

Alternative Names

Hepatocellular carcinoma autoantigen p62 ELISA Kit; IF2B2_HUMAN ELISA Kit; IGF 2 mRNA binding protein 2 ELISA Kit; IGF II mRNA binding protein 2 ELISA Kit; IGF-II mRNA-binding protein 2 ELISA Kit; IGF2 mRNA-binding protein 2 ELISA Kit; Igf2bp2 ELISA Kit; IMP 2 ELISA Kit; IMP-2 ELISA Kit; IMP2 ELISA Kit; Insulin like growth factor 2 mRNA binding protein 2 ELISA Kit; Insulin-like growth factor 2 mRNA-binding protein 2 ELISA Kit; OTTHUMP00000082606 ELISA Kit; OTTHUMP00000082607 ELISA Kit; OTTHUMP00000082608 ELISA Kit; p62 ELISA Kit; VICKZ 2 ELISA Kit; VICKZ family member 2 ELISA Kit; VICKZ2 ELISA Kit

Abbreviation

IGF2BP2

Uniprot No.

Q9Y6M1

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGF2BP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	96
	Range %	92-100
1:4	Average %	90
	Range %	85-94
1:8	Average %	104
	Range %	100-108

Recovery

The recovery of human IGF2BP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	95	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.167	2.249	2.208	2.051
4000	1.649	1.703	1.676	1.519
2000	1.174	1.114	1.144	0.987
1000	0.791	0.758	0.775	0.618
500	0.458	0.472	0.465	0.308
250	0.335	0.347	0.341	0.184
125	0.226	0.236	0.231	0.074
0	0.153	0.160	0.157

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human IGF2BP2 ELISA Kit was designed for the quantitative measurement of Human IGF2BP2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

Target Background

Function
(From Uniprot)

RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts.

Gene References into Functions

IGF2BP2 protein is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1. PMID: 28753127
The miR-485-5p/IGF2BP2 axis orchestrates the growth and metastasis of non-small cell lung cancer. PMID: 29510198
IGF2BP2/IMP2 expression is linked to short survival and metastasis in esophageal adenocarcinoma PMID: 27391348
insulin-like growth factor 2 mRNA-binding protein 2 (IMP2) binds to let-7 miRNA recognition elements (MREs) and prevents let-7 target gene silencing. PMID: 27184842
miR-1193 suppressed proliferation and invasion of human breast cancer cells via translational suppression of IGF2BP2. PMID: 27733218
Our data suggests that IGF2BP2 polymorphisms are associated with Type 2 Diabetes Mellitus in Asian populations. PMID: 27294943
Study conclude that integration of genomic and transcriptomic data implicate circulating IGF2BP2 mRNA levels associated with glucose and insulin homeostasis PMID: 27625022
IGF2BP2 as a post-transcriptional regulatory mRNA-binding factor that contributes to Colorectal carcinogenesis. PMID: 27153315
p62/IMP2 stimulates cell migration and reduces cell adhesion, contributing to breast cancer progression. PMID: 26416451
IMP2 expression is higher in ovarian and endometrial high-grade serous carcinomas (HGSC) than in ovarian or endometrial endometrioid carcinoma. Knockdown in ovarian HGSC cell line decreased cell proliferation. PMID: 26889980
Our results suggest IGF2BP2 and KCNQ1 polymorphisms might be independent predictors of chemotherapeutic response PMID: 26115082
HPV16 Down-Regulates the Insulin-Like Growth Factor Binding Protein 2 to Promote Epithelial Invasion PMID: 26107517
Imp2 regulates the activity of IGF2, which further activates PI3K/Akt signaling. PMID: 25719943
The IGF2BP2 gene rs1470579 and rs4402960 polymorphisms were associated with type 2 diabetes patients and therapeutic efficacy of pioglitazone in this Chinese population PMID: 25247335
The IGF2BP2 gene rs4402960 polymorphism increases the breast cancer risk of Chinese females with Han nationality, and is a breast cancer predisposing gene. PMID: 25661373
rs4402960 of IGF2BP2 gene is a strong candidate for Type 2 diabetes susceptibility and overweight/obesity risk. PMID: 24636221
Data suggest that autoantibody against IGF2 mRNA-Binding Protein 2 (IMP2/p62) may be a useful serum biomarker for early-stage breast cancer screening and diagnosis. PMID: 25721883
The case-control study and meta-analysis revealed a significant association between the IGF2BP2 rs4402960 variant and type 2 diabetes in Moroccan and Arab populations.[meta-analysis] PMID: 24898818
The present study provided data suggesting that the wild C allele of IGF2BP2 (rs4402960) had a protective effect against T2DM in obese subjects of Chinese Han population. PMID: 25062844
VICKZ1 and VICKZ2 are overexpressed in ovarian carcinoma effusions suggesting a biological role at this anatomical site, and appear to have a role in proteolysis and invasion. VICKZ2 may be a prognostic marker in ovarian serous carcinoma effusions. PMID: 24814803
IGF2BP2 was strongly associated with the risk of T2DM in Chinese Han population. PMID: 24229666
Our results revealed two novel genes (IGF2BP2 and TNFRSF13B), whose function could account for the biologic pathways influencing MetS phenotypes. PMID: 23418049
Polymorphisms in PPARgamma(2) and IGF2BP2 were shown to be highly correlated with GDM occurrence, whereas no correlation was found for KCNQ1 polymorphisms. PMID: 23364967
TCF7L2 was replicated in this study (P = 0.004; combined analysis P = 3.8 x 10(-6)), and type 2 diabetes SNPs at or near CDKAL1, CDKN2A/B, and IGF2BP2 were associated with CFRD PMID: 23670970
IGF2BP2 genetic variants contribute to insulin resistance in Russian NIDDM patients. PMID: 23403707
findings implicate the HMGA2-IGFBP2-NRAS signaling pathway as a critical oncogenic driver in embryonic rhabdomyosarcoma PMID: 23536553
IGF2BP2 may play a role in susceptibility to schizophrenia in Han Chinese, supporting the hypothesis that the co-occurrence of type 2 diabetes mellitus and schizophrenia may be explained by shared genetic risk variants. PMID: 23656854
rs4402960 and rs1470579 lymorphisms of the IFG2BP2po is a risk factor for developing type 2 diabetes. PMID: 22015911
there is a strong immune response of anti-p62 in sera from patients with colon cancer compared with normal human sera (NHS). PMID: 23421499
p62 exerts IGF2-independent antiapoptotic action, which is facilitated via phosphorylation of ERK1/2. PMID: 23257922
IGF2BP2 alternative variants were associated with GADA negative diabetes. The IGF2BP2 haplotypes and diplotypes increased the risk of diabetes in Malaysian subject. PMID: 23029108
In African Americans, seven of the 29 SNPs examined were found to be associated with T2D risk at P PMID: 23144361
genetic association studies: Data suggest that an SNP in IGF2BP2 (rs4402960) is associated with type 2 diabetes; IGF2BP2 may have genetic interactions with insulin-like growth factor II with a protective effect in male patients with type 1 diabetes. PMID: 22770937
Association to type 2 diabetes was found for rs13266634 (SLC30A8), rs7923837 (HHEX), rs10811661 (CDKN2A/2B), rs4402960 (IGF2BP2), rs12779790 (CDC123/CAMK1D), and rs2237892 (KCNQ1). PMID: 22923468
oncofetal insulin-like growth factor 2 mRNA-binding protein 2 (IMP2, IGF2BP2) regulates oxidative phosphorylation (OXPHOS) in primary glioblastoma (GBM) sphere cultures (gliomaspheres) PMID: 22899010
Data validate that IGF2BP2 susceptibility variants rs4402960 and rs1470579 associate with T2DM in Lebanese Arabs. PMID: 22245690
Two isoforms of the mRNA binding protein IGF2BP2 are generated by alternative translational initiation. PMID: 22427968
IGF2BP2 genetic variation is associated with type 2 diabetes. PMID: 22032244
Six SNP(rs7754840 in CDKAL1, rs391300 in SRR, rs2383208 in CDKN2A/2B, rs4402960 in IGF2BP2, rs10830963 in MTNR1B, rs4607517 in GCK)risk alleles of type 2 diabetes were associated with GDM in pregnant Chinese women. PMID: 22096510
meta-analysis suggested that IGF2BP2 rs4402960 polymorphism conferred elevated risk of T2DM, especially in European, East Asian and South Asian populations PMID: 21839790
involved in the selective autophagic clearance of non-ubiquitylated aggregation-prone substrates PMID: 21771882
IGF2 is emerging as an important gene for ovarian cancer. PMID: 21422097
The induction of a steatotic phenotype implies that p62 plays a role in hepatic pathophysiology PMID: 21145819
Double phosphorylation promotes IMP2 binding to the IGF2 leader 3 mRNA 5' untranslated region, and the translational initiation of this mRNA PMID: 21576258
non-replications of IGF2BP2 associations with type 2 diabetes PMID: 20627640
Data reveal how the posttranscriptional regulation of gene expression by IMP-2 contributes to the control of adhesion structures and stable microtubules and demonstrate an important function for IMP-2 in cellular motility. PMID: 20956565
findings show that IGF2BP2 rs1470579 and rs4402960 polymorphisms may be associated with the development of type 2 diabetes and these polymorphisms may affect the therapeutic efficacy of repaglinide in Chinese T2DM patientsmellitus PMID: 20523342
prostate cancer was inversely associated with the IGF2BP2 rs4402960 T allele PMID: 20142250
HMGA2 differentially regulates expression of IMP family members during mouse embryogenesis. PMID: 15225648
Study suggests that there is a significant association between expression of IMP-2 and the growth of tumor cells, in which IMP-2 is associated with apoptosis induced by tretinoin. PMID: 15618018

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Subcellular Location

Nucleus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. In response to cellular stress, such as oxidative stress, recruited to stress granules.

Protein Families

RRM IMP/VICKZ family

Tissue Specificity

Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, ki

Database Links

HGNC: 28867

OMIM: 608289

KEGG: hsa:10644

STRING: 9606.ENSP00000371634

UniGene: Hs.293396

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Code	CSB-EL011091HU
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Human Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2) ELISA kit

Product Details

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