Human Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2) ELISA kit

Code CSB-EL011091HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9Y6M1
Lead Time 5-7 working days
Abbreviation IGF2BP2
Protein Biological Process 1 Transcription/Transcription regulation
Target Name insulin-like growth factor 2 mRNA binding protein 2
Alias IMP-2, IMP2, VICKZ2, p62, IGF-II mRNA-binding protein 2
Species Homo sapiens (Human)
Protein Biological Process 3 Translation regulation
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. This protein contains several four KH domains and two RRM domains. It functions by binding to the 5 UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
HGNC 28867
RGD 1305614
MGI 1890358
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IGF2BP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 80-90
1:2 Average % 96
Range % 92-100
1:4 Average % 90
Range % 85-94
1:8 Average % 104
Range % 100-108
Recovery
The recovery of human IGF2BP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 84-93
EDTA plasma (n=4) 95 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
8000 2.167 2.249 2.208 2.051
4000 1.649 1.703 1.676 1.519
2000 1.174 1.114 1.144 0.987
1000 0.791 0.758 0.775 0.618
500 0.458 0.472 0.465 0.308
250 0.335 0.347 0.341 0.184
125 0.226 0.236 0.231 0.074
0 0.153 0.160 0.157  
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Function RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts.
Subcellular Location Nucleus, Cytoplasm
Protein Families RRM IMP/VICKZ family
Tissue Specificity Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, ki
Database Links

HGNC: 28867

OMIM: 608289

KEGG: hsa:10644

STRING: 9606.ENSP00000371634

UniGene: Hs.293396

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