Target Name
insulin-like 5
Alternative Names
INSL5 ELISA Kit; INSL5_HUMAN ELISA Kit; Insulin like peptide INSL5 ELISA Kit; Insulin-like peptide 5 ELISA Kit; Insulin-like peptide INSL5 A chain ELISA Kit; MGC126695 ELISA Kit; MGC126697 ELISA Kit; PRO182 ELISA Kit; RIF2 ELISA Kit; RP24-72J16.1 ELISA Kit; UNQ156 ELISA Kit; UNQ156/PRO182 ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
8 ng/mL-140 ng/mL
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human INSL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 93 | | |
| Range % | 85-103 | | |
| 1:2 | Average % | 96 | | |
| Range % | 89-105 | | |
| 1:4 | Average % | 90 | | |
| Range % | 86-97 | | |
| 1:8 | Average % | 93 | | |
| Range % | 91-98 | | |
Recovery
| The recovery of human INSL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 96 | 90-102 | | |
| EDTA plasma (n=4) | 94 | 88-100 | | |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | ng/ml | OD1 | OD2 | Average | | | | | 140 | 1.586 | 1.597 | 1.592 | | | | | 80 | 0.943 | 0.955 | 0.949 | | | | | 32 | 0.365 | 0.371 | 0.368 | | | | | 16 | 0.175 | 0.184 | 0.180 | | | | | 8 | 0.101 | 0.105 | 0.103 | | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
