Human Interferon epsilon(IFNE) ELISA kit

Code CSB-EL011049HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
interferon, epsilon
Alternative Names
IFNE ELISA Kit; IFNE1 ELISA Kit; UNQ360/PRO655 ELISA Kit; Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit
Abbreviation
IFNE
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 85-95  
1:2 Average % 93  
Range % 88-99  
1:4 Average % 92  
Range % 87-100  
1:8 Average % 95  
Range % 88-99  
Recovery
The recovery of human IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 91-100  
EDTA plasma (n=4) 93 88-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.058 2.011 2.035 1.931  
500 1.664 1.547 1.606 1.502  
250 1.188 1.149 1.169 1.065  
125 0.739 0.704 0.722 0.618  
62.5 0.561 0.521 0.541 0.437  
31.2 0.375 0.354 0.365 0.261  
15.6 0.219 0.211 0.215 0.111  
0 0.105 0.103 0.104    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human IFNE ELISA Kit was designed for the quantitative measurement of Human IFNE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral and bacterial genital infections.
Gene References into Functions
  1. IFN epsilon protects macrophages against HIV infection through a type I IFN independent mechanism. PMID: 27942584
  2. human IFNvarepsilon suppresses HIV replication at multiple stages of infection. PMID: 28045025
  3. female sex workers decreased susceptibility to HIV-1 infection also have increased levels of IFNE gene and protein expression in the cervical epithelium PMID: 26555708
  4. T Allele of nonsense polymorphism (rs2039381, Gln71Stop) of interferon-epsilon is a risk factor for the development of intracerebral hemorrhage. PMID: 24055696
  5. Full-length IFN-epsilon 5'UTR markedly suppressed mRNA expression under basal conditions. It contains 2 stable stem-loop structures which associate with importin 9. Loop 1 is essential for regulation of mRNA expression. PMID: 23851686
  6. genetic polymorphism is related to onset time of vitiligo in Korean patients PMID: 23802172
  7. Observational study of gene-disease association. (HuGE Navigator) PMID: 20574843
  8. Observational study of gene-disease association. (HuGE Navigator) PMID: 20588308
  9. Observational study of gene-disease association. (HuGE Navigator) PMID: 20601674
  10. The structue and mRNA expression pattern of IFN-epsilon1 suggest that it may have a function distinct from those other members of type I INF. PMID: 15233997
  11. TNF-alpha leads to stabilization of IFN-epsilon mRNA, increased IFN-epsilon synthesis, engagement of type I IFNRs, increased STAT1 expression and phosphorylation, and up-regulation of retinoic acid-inducible gene-I expression PMID: 17878351
  12. A meta-analysis and a single-nucleotide polymorphism (SNP) rs1333049 representing the 9p21.3 locus provide unprecedented evidence for association between genetic variants at chromosome 9p21.3 and risk of coronary artery disease. PMID: 18362232
  13. In macrophages IFN-tau increased the synthesis of 2',5'-oligoadenylate synthetase/RNase L, MxA protein, IL-10 & IL-6, but not of IL-1ss or TNF-alpha. PMID: 18842358

Show More

Hide All

Subcellular Location
Secreted.
Protein Families
Alpha/beta interferon family
Tissue Specificity
Endometrium-specific.
Database Links

HGNC: 18163

OMIM: 615223

KEGG: hsa:338376

STRING: 9606.ENSP00000418018

UniGene: Hs.682604

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X