Product Details

Target Name

interferon, kappa

Alternative Names

IFN kappa ELISA Kit; IFN-kappa ELISA Kit; Ifnk ELISA Kit; IFNK_HUMAN ELISA Kit; Interferon kappa ELISA Kit

Abbreviation

IFNK

Uniprot No.

Q9P0W0

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IFNK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	85
	Range %	80-90
1:4	Average %	90
	Range %	85-94
1:8	Average %	98
	Range %	94-103

Recovery

The recovery of human IFNK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	97-105
EDTA plasma (n=4)	92	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.694	2.758	2.726	2.622
500	2.309	2.182	2.246	2.142
250	1.515	1.601	1.558	1.454
125	0.954	1.004	0.979	0.875
62.5	0.521	0.560	0.541	0.437
31.25	0.287	0.293	0.290	0.186
15.6	0.193	0.205	0.199	0.095
0	0.100	0.107	0.104

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human IFNK ELISA Kit was designed for the quantitative measurement of Human IFNK protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Related Products

Target Background

Function
(From Uniprot)

May play a role in the regulation of immune cell function. Cytokine that imparts cellular protection against viral infection in a species-specific manner. Activates the interferon-stimulated response element signaling pathway. It is able to directly modulate cytokine release from monocytes and dendritic cells. Binds heparin.

Gene References into Functions

investigation of common variable immunodeficiency (CVID) from 2 German families; report on the occurrence of a common and one novel truncating IFNK mutation in cases with CVID; the frequency distribution of c.30_31insTGTT in cases and controls as well as the observed segregation patterns in CVID families exclude IFNK mutations as major risk factor in CVID PMID: 28324805
These results suggest that high-risk human papillomavirus 31 target interferon kappa to prevent Sp100 expression and identify Sp100 as an interferon-stimulated gene with anti-human papillomavirus activity. PMID: 26491169
This study demonstrates that E2 proteins of high risk human papillomavirus reduce STING and IFN-kappa transcription. PMID: 24614210
The viral E6 and E7 oncogenes are sufficient for interferon-kappa repression, with E6 being mainly responsible. PMID: 21849431
IFN-kappa is down-regulated in cervical keratinocytes harboring HPV, which may be a contributing factor in the progression of a cervical lesion PMID: 20479716
Studied IFNK single nucleotide polymorphisms in 3982 Systemic lupus erythematosus cases and 4275 controls. PMID: 20706608
IFN-kappa is able to directly modulate cytokine release from monocytes and dendritic cells, inhibit inducible IL-12 release from monocytes, and bind strongly to heparin. PMID: 12391192
This is the first report showing an epigenetic silencing of type I IFN after HPV16 oncogene expression PMID: 19887612

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Subcellular Location

Secreted.

Protein Families

Alpha/beta interferon family

Tissue Specificity

Expressed in keratinocytes, monocytes and in resting dendritic cells.

Database Links

HGNC: 21714

OMIM: 615326

KEGG: hsa:56832

STRING: 9606.ENSP00000276943

UniGene: Hs.591083

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Code	CSB-EL011053HU
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Human Interferon kappa(IFNK) ELISA kit

Product Details

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Target Background