Human Interleukin 16,IL-16 ELISA KIT

Code CSB-E04605h
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
interleukin 16 (lymphocyte chemoattractant factor)
Alternative Names
HGNC:5980 ELISA Kit; HsT19289 ELISA Kit; IL-16 ELISA Kit; IL16 ELISA Kit; IL16_HUMAN ELISA Kit; Interleukin 16 precursor ELISA Kit; Interleukin-16 ELISA Kit; LCF ELISA Kit; Lymphocyte chemoattractant factor ELISA Kit; Neuronal interleukin 16 ELISA Kit; NIL16 ELISA Kit; prIL 16 ELISA Kit; PrIL16 ELISA Kit; Prointerleukin 16 ELISA Kit
Abbreviation
IL16
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 88
Range % 83-97
1:2 Average % 97
Range % 91-104
1:4 Average % 84
Range % 81-88
1:8 Average % 89
Range % 81-96
Recovery
The recovery of human IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 94-101
EDTA plasma (n=4) 94 90-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 1.774 1.848 1.811 1.644
5 1.003 1.014 1.009 0.842
2.5 0.666 0.687 0.677 0.510
1.25 0.401 0.412 0.407 0.240
0.625 0.289 0.287 0.288 0.121
0.312 0.245 0.253 0.249 0.082
0.156 0.211 0.224 0.218 0.051
0 0.163 0.170 0.167  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The ELISA Kit is designed for quantitatively measuring human IL-16 levels in samples, including serum, plasma, cell culture supernates, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of IL-16 in samples. The color intensity is measured at 450 nm via a microplate reader.

IL-16 is known to induce the IL-2Rα on T cells, suppress HIV replication and inhibit T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions. In addition, IL-16 acts in concert with IL-2 and/or IL-15 to promote CD4+ T-cell proliferation, and is a chemoattractant factor for CD4+ cells. IL-16 plays a key role in airway hyper-responsiveness and up-regulation of IgE. IL-16 has been associated with diseases characterized by CD4+T-cell involvement, such as allergic asthma, rheumatoid arthritis, multiple sclerosis, and acquired immunodeficiency syndrome.

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Target Background

Function
(From Uniprot)
Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.; may act as a scaffolding protein that anchors ion channels in the membrane.; Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
Gene References into Functions
  1. rs11556218 is associated with endometriosis in Greek women, probably by resulting in the aberrant expression of IL16 PMID: 29328375
  2. There was a decreased frequency of IL16 rs4778889 CC genotype carriers among women with gestational diabetes mellitus, however, there was no significant difference in the distribution of alleles. PMID: 28580570
  3. Serum IL-6, serum YKL-40, and plasma VEGF were significantly correlated with disease activity at baseline in early rheumatoid arthritis. PMID: 29336711
  4. serum IL-16 level may not only serve as a useful diagnostic biomarker for patients with GC, but also may predict cancer recurrence and patient prognosis. PMID: 28990054
  5. Association of interleukin 16 gene polymorphisms and plasma IL16 level with osteosarcoma risk. PMID: 27703190
  6. Results suggest that IL16 genotype predict higher risk of death and progression, respectively, in NSCLC patients PMID: 28807247
  7. rs11556218 T/G genotype associated with increased risk for development of osteoporosis in Chinese postmenopausal women PMID: 27984748
  8. Data indicate an association between interleukin-16 (IL-16) rs4072111 and rs1131445 with progression to the severe stages (III-IV) of endometriosis. PMID: 27484651
  9. Long-term exposure to tobacco smoke does not markedly alter extracellular concentrations of IL-16 protein in blood. However, it does decrease the intracellular IL-16 concentrations in blood NK cells. PMID: 27695312
  10. data revealed an association between circulating IL-16 and severity of psoriasis which indicates that this cytokine could serve as a potential marker of disease activity. However, further investigations are required PMID: 27788245
  11. Structure of a Potential Therapeutic Antibody Bound to Interleukin-16 PMID: 27231345
  12. No significant differences were observed in rs11556218 genotype frequencies between the study groups. In conclusion, the results of our study reveal an association between the IL-16 rs4778889 polymorphism and heightened risk of renal cell cancer . PMID: 27323152
  13. We found that the rs1131445 T/C and rs4072111 T/C variants of IL-16 were significantly associated with increased risk of gastric cancer in Iranian population. PMID: 26346169
  14. the results of our study suggested an association between the IL-16 rs4778889 polymorphism and an elevated risk of renal cell carcinoma PMID: 26823871
  15. We observed highly significant reductions in the concentration of circulating interleukin (IL)-16, IL-7, and Vascular Endothelial Growth Factor A (VEGF-A) in encephalomyelitis/chronic fatigue syndrome patients. PMID: 26615570
  16. IL-16 rs1131445 C/T polymorphism is related to the susceptibility to cancer in Asians (meta-analysis) PMID: 26842762
  17. The IL16 rs4072111 polymorphism may be associated with susceptibility to AD and the T allele may have a protective role in the progression of AD in an Iranian population. PMID: 26386715
  18. intrathyroidal production of IL-14 and IL-16 associated with the pathogenesis of autoimmune thyroid disease PMID: 25940130
  19. the lack of a relationship between genotype and serum levels of IL-16 seems to suggest a more complex regulation of this cytokine at the post-translational level and/or the interplay with other cytokines. PMID: 26154181
  20. The results suggested that the variants in IL-16 gene rs11556218 site were associated with a decreased knee osteoarthritis risk after adjusting for age, sex, BMI, and smoking and drinking status. PMID: 25954818
  21. Data suggest that thymic stromal lymphopoietin (TSLP) and interleukin-16 (IL-16), expressed at early stages of disease, function to recruit malignant T cells to the skin. PMID: 24794807
  22. The production of IL-16 contributed to lung damage as neutralization of IL-16 enhanced S. aureus clearance and resulted in diminished lung pathology in S. aureus pneumonia. PMID: 24736233
  23. The findings suggest that the IL-16 rs 11556218 polymorphism may be used as a susceptibility marker for glioma. PMID: 25166752
  24. IL-16 rs11556218 T/G was associated with a significant increased risk of cancer. PMID: 24969906
  25. The rs11556218 T/G polymorphism of the IL-16 gene was significantly associated with elevated cancer risk in Asian populations. [meta-analysis] PMID: 24328527
  26. IL16 polymorphisms may play a role in the pathophysiology of alopecia areata or in the expression of phenotypes in Koreans. PMID: 24320753
  27. IL-16 rs4778889 T/C polymorphism is not associated with cancer. PMID: 24568499
  28. The IL-16 SNP rs8028364 was found to be associated with Graves' disease(GD) when compared with the control subjects. PMID: 24694201
  29. An association between decreased plasma IL-16 and emphysema susceptibility. PMID: 24138069
  30. Rs4778889 CC and rs11556218 GG genotype was significantly associated with 1.97 and 1.84-fold increased risk of non-cardia gastric cancer. PMID: 24175812
  31. the IL-16 polymorphism may be related to the etiology of ischemic stroke in the Chinese population PMID: 24288444
  32. This study showed that tissue expression and serum levels of IL-16 increase in association with malignant ovarian tumor development and progression. PMID: 24380743
  33. IL-16 plays an important role in the proliferation and apoptosis of MT-4 cells PMID: 24200060
  34. Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction. PMID: 23894370
  35. The IL-16 SNPs rs8034928 and rs11556218 are associated with the coronary artery disease risk in the Chinese population. PMID: 23881440
  36. It is feasible to predict preterm birth by measuring the amniotic fluid levels of IL-16 especially for the pregnant women requiring genetic amniocentesis during early second trimester. PMID: 23020666
  37. Authors identified three independent loci within IL16 that were associated with prostate cancer risk. SNP expression quantitative trait loci analyses revealed that rs7175701 is predicted to influence the expression of IL16 and other cancer-related genes. PMID: 22923025
  38. study identified IL-16 as a critical tristetraprolin-regulated chemotactic factor that contributes to monocytes/macrophages migration PMID: 23241166
  39. a significant relationship exists between the miRNA-binding site polymorphism of the IL-16 gene and colorectal cancer risk in the Iranian population PMID: 22939228
  40. IL-16 was strongly overexpressed in the bone marrow of myeloma patients compared with healthy donors. IL-16 is an important growth-promoting factor in multiple myeloma. PMID: 22745469
  41. IL-16 rs11556218 G/T polymorphism is significantly associated with the risk of coronary artery disease in the Chinese Han population. PMID: 21703255
  42. Data show that IL16 single nucleotide polymorphisms are associated with chronic hepatitis B virus infection and hepatocellular carcinoma. PMID: 22019522
  43. Serum IL-16 levels of atopic dermatitis patients correlate to some extent with sensitization. PMID: 21447961
  44. Our results demonstrate that loss of intracellular IL-16 in peripheral blood T cells corresponds to lymphoma stage in Sezary Syndrome patients and orrelates with loss of surface CD26. PMID: 20878214
  45. TcdA and TcdB downregulate IL-16 gene expression via inhibition of Rho GTPases.(TcdA and TcdB from C. difficile strain VPI 10463) PMID: 21267712
  46. The TG/GG genotypes of rs11556218 T/G were associated with a significantly increased risk of coronary artery disease as compared with the TT genotype. PMID: 21214401
  47. Ability of IL-16 homolog from leech Hirudo medicinalis to recruit microglial cells to sites of nervous system injury suggests its in the crosstalk between neurons and microglia in the leech central nervous system repair. PMID: 20578037
  48. Tobacco smoke depletes preformed intracellular IL-16 protein, inhibits its de novo synthesis, and distorts key cellular functions in human CD8(+) cells. PMID: 21036918
  49. the rs4778889 T/C polymorphism of the IL-16 gene may be associated with risk of endometriosis PMID: 20662556
  50. IL-16 -295 T>C polymorphism is significantly associated with a higher risk of developing RCC in Chinese population. PMID: 20529140

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Subcellular Location
[Interleukin-16]: Secreted.; [Isoform 1]: Cytoplasm.; [Isoform 3]: Cytoplasm. Nucleus.
Tissue Specificity
[Isoform 3]: Expressed in hemopoietic tissues, such as resting T-cells, but undetectable during active T-cell proliferation.
Database Links

HGNC: 5980

OMIM: 603035

KEGG: hsa:3603

STRING: 9606.ENSP00000302935

UniGene: Hs.459095

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