Product Details

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/ml, corresponding to a specific activity of >1.0x10⁹ IU/mg.

Target Names

IL16

Uniprot No.

Q14005

Research Area

Immunology

Alternative Names

HGNC:5980; HsT19289; IL-16; IL16; IL16_HUMAN; Interleukin 16 precursor; Interleukin-16; LCF; Lymphocyte chemoattractant factor; Neuronal interleukin 16; NIL16; prIL 16; PrIL16 ; Prointerleukin 16

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1212-1332aa

Complete Sequence

SAASASAASD VSVESTAEAT VCTVTLEKMS AGLGFSLEGG KGSLHGDKPL TINRIFKGAA SEQSETVQPG DEILQLGGTA MQGLTRFEAW NIIKALPDGP VTIVIRRKSL QSKETTAAGD S

Mol. Weight

12.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

IL-16 functions as a CD4-selective chemoattractant cytokine that bridges innate and adaptive immunity, and this tag-free recombinant form faithfully represents the mature 1212–1332 aa sequence, ensuring native-like receptor engagement. Biological activity has been confirmed through a T lymphocyte chemotaxis bioassay, with functional potency observed at 50–500 ng/ml using human peripheral T cells—data that supports use in migration assays, CD4⁺ T cell activation studies, and downstream signaling investigations involving p56lck and PI3K pathways. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that commonly confound immune cell functional readouts, making this protein appropriate for sensitive in vivo inflammation models and primary cell-based assays where cytokine-specific effects must be cleanly resolved. Purity exceeding 95% by SDS-PAGE, combined with this stringent endotoxin profile, also provides a suitable basis for use as a reference standard in cytokine detection assays or as a positive control in ELISA and western blot validation workflows.

Target Background

Function

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.; may act as a scaffolding protein that anchors ion channels in the membrane.; Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

Gene References into Functions

rs11556218 is associated with endometriosis in Greek women, probably by resulting in the aberrant expression of IL16 PMID: 29328375
There was a decreased frequency of IL16 rs4778889 CC genotype carriers among women with gestational diabetes mellitus, however, there was no significant difference in the distribution of alleles. PMID: 28580570
Serum IL-6, serum YKL-40, and plasma VEGF were significantly correlated with disease activity at baseline in early rheumatoid arthritis. PMID: 29336711
serum IL-16 level may not only serve as a useful diagnostic biomarker for patients with GC, but also may predict cancer recurrence and patient prognosis. PMID: 28990054
Association of interleukin 16 gene polymorphisms and plasma IL16 level with osteosarcoma risk. PMID: 27703190
Results suggest that IL16 genotype predict higher risk of death and progression, respectively, in NSCLC patients PMID: 28807247
rs11556218 T/G genotype associated with increased risk for development of osteoporosis in Chinese postmenopausal women PMID: 27984748
Data indicate an association between interleukin-16 (IL-16) rs4072111 and rs1131445 with progression to the severe stages (III-IV) of endometriosis. PMID: 27484651
Long-term exposure to tobacco smoke does not markedly alter extracellular concentrations of IL-16 protein in blood. However, it does decrease the intracellular IL-16 concentrations in blood NK cells. PMID: 27695312
data revealed an association between circulating IL-16 and severity of psoriasis which indicates that this cytokine could serve as a potential marker of disease activity. However, further investigations are required PMID: 27788245
Structure of a Potential Therapeutic Antibody Bound to Interleukin-16 PMID: 27231345
No significant differences were observed in rs11556218 genotype frequencies between the study groups. In conclusion, the results of our study reveal an association between the IL-16 rs4778889 polymorphism and heightened risk of renal cell cancer . PMID: 27323152
We found that the rs1131445 T/C and rs4072111 T/C variants of IL-16 were significantly associated with increased risk of gastric cancer in Iranian population. PMID: 26346169
the results of our study suggested an association between the IL-16 rs4778889 polymorphism and an elevated risk of renal cell carcinoma PMID: 26823871
We observed highly significant reductions in the concentration of circulating interleukin (IL)-16, IL-7, and Vascular Endothelial Growth Factor A (VEGF-A) in encephalomyelitis/chronic fatigue syndrome patients. PMID: 26615570
IL-16 rs1131445 C/T polymorphism is related to the susceptibility to cancer in Asians (meta-analysis) PMID: 26842762
The IL16 rs4072111 polymorphism may be associated with susceptibility to AD and the T allele may have a protective role in the progression of AD in an Iranian population. PMID: 26386715
intrathyroidal production of IL-14 and IL-16 associated with the pathogenesis of autoimmune thyroid disease PMID: 25940130
the lack of a relationship between genotype and serum levels of IL-16 seems to suggest a more complex regulation of this cytokine at the post-translational level and/or the interplay with other cytokines. PMID: 26154181
The results suggested that the variants in IL-16 gene rs11556218 site were associated with a decreased knee osteoarthritis risk after adjusting for age, sex, BMI, and smoking and drinking status. PMID: 25954818
Data suggest that thymic stromal lymphopoietin (TSLP) and interleukin-16 (IL-16), expressed at early stages of disease, function to recruit malignant T cells to the skin. PMID: 24794807
The production of IL-16 contributed to lung damage as neutralization of IL-16 enhanced S. aureus clearance and resulted in diminished lung pathology in S. aureus pneumonia. PMID: 24736233
The findings suggest that the IL-16 rs 11556218 polymorphism may be used as a susceptibility marker for glioma. PMID: 25166752
IL-16 rs11556218 T/G was associated with a significant increased risk of cancer. PMID: 24969906
The rs11556218 T/G polymorphism of the IL-16 gene was significantly associated with elevated cancer risk in Asian populations. [meta-analysis] PMID: 24328527
IL16 polymorphisms may play a role in the pathophysiology of alopecia areata or in the expression of phenotypes in Koreans. PMID: 24320753
IL-16 rs4778889 T/C polymorphism is not associated with cancer. PMID: 24568499
The IL-16 SNP rs8028364 was found to be associated with Graves' disease(GD) when compared with the control subjects. PMID: 24694201
An association between decreased plasma IL-16 and emphysema susceptibility. PMID: 24138069
Rs4778889 CC and rs11556218 GG genotype was significantly associated with 1.97 and 1.84-fold increased risk of non-cardia gastric cancer. PMID: 24175812
the IL-16 polymorphism may be related to the etiology of ischemic stroke in the Chinese population PMID: 24288444
This study showed that tissue expression and serum levels of IL-16 increase in association with malignant ovarian tumor development and progression. PMID: 24380743
IL-16 plays an important role in the proliferation and apoptosis of MT-4 cells PMID: 24200060
Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction. PMID: 23894370
The IL-16 SNPs rs8034928 and rs11556218 are associated with the coronary artery disease risk in the Chinese population. PMID: 23881440
It is feasible to predict preterm birth by measuring the amniotic fluid levels of IL-16 especially for the pregnant women requiring genetic amniocentesis during early second trimester. PMID: 23020666
Authors identified three independent loci within IL16 that were associated with prostate cancer risk. SNP expression quantitative trait loci analyses revealed that rs7175701 is predicted to influence the expression of IL16 and other cancer-related genes. PMID: 22923025
study identified IL-16 as a critical tristetraprolin-regulated chemotactic factor that contributes to monocytes/macrophages migration PMID: 23241166
a significant relationship exists between the miRNA-binding site polymorphism of the IL-16 gene and colorectal cancer risk in the Iranian population PMID: 22939228
IL-16 was strongly overexpressed in the bone marrow of myeloma patients compared with healthy donors. IL-16 is an important growth-promoting factor in multiple myeloma. PMID: 22745469
IL-16 rs11556218 G/T polymorphism is significantly associated with the risk of coronary artery disease in the Chinese Han population. PMID: 21703255
Data show that IL16 single nucleotide polymorphisms are associated with chronic hepatitis B virus infection and hepatocellular carcinoma. PMID: 22019522
Serum IL-16 levels of atopic dermatitis patients correlate to some extent with sensitization. PMID: 21447961
Our results demonstrate that loss of intracellular IL-16 in peripheral blood T cells corresponds to lymphoma stage in Sezary Syndrome patients and orrelates with loss of surface CD26. PMID: 20878214
TcdA and TcdB downregulate IL-16 gene expression via inhibition of Rho GTPases.(TcdA and TcdB from C. difficile strain VPI 10463) PMID: 21267712
The TG/GG genotypes of rs11556218 T/G were associated with a significantly increased risk of coronary artery disease as compared with the TT genotype. PMID: 21214401
Ability of IL-16 homolog from leech Hirudo medicinalis to recruit microglial cells to sites of nervous system injury suggests its in the crosstalk between neurons and microglia in the leech central nervous system repair. PMID: 20578037
Tobacco smoke depletes preformed intracellular IL-16 protein, inhibits its de novo synthesis, and distorts key cellular functions in human CD8(+) cells. PMID: 21036918
the rs4778889 T/C polymorphism of the IL-16 gene may be associated with risk of endometriosis PMID: 20662556
IL-16 -295 T>C polymorphism is significantly associated with a higher risk of developing RCC in Chinese population. PMID: 20529140

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Subcellular Location

[Interleukin-16]: Secreted.; [Isoform 1]: Cytoplasm.; [Isoform 3]: Cytoplasm. Nucleus.

Tissue Specificity

[Isoform 3]: Expressed in hemopoietic tissues, such as resting T-cells, but undetectable during active T-cell proliferation.

Database Links

HGNC: 5980

OMIM: 603035

KEGG: hsa:3603

STRING: 9606.ENSP00000302935

UniGene: Hs.459095

Code	CSB-AP001641HU
Abbreviation	Recombinant Human IL16 protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Recombinant Human interleukin-16 proein (IL16) (Active)

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