Human Interleukin-22 receptor subunit alpha-2(IL22RA2) ELISA kit

Code CSB-EL011637HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
interleukin 22 receptor, alpha 2
Alternative Names
IL 22BP ELISA Kit; IL 22RA2 ELISA Kit; IL22RA2 ELISA Kit; Class II cytokine receptor ELISA Kit; CRF2 10 ELISA Kit; CRF2 S1 ELISA Kit; CRF2-10 ELISA Kit; CRF2-S1 ELISA Kit; CRF2X ELISA Kit; Cytokine receptor class II member 10 ELISA Kit; Cytokine receptor class-II member 10 ELISA Kit; Cytokine receptor family 2 member 10 ELISA Kit; Cytokine receptor family type 2 ELISA Kit; Cytokine receptor family type 2 soluble 1 ELISA Kit; I22R2_HUMAN ELISA Kit; IL 22 receptor subunit alpha 2 ELISA Kit; IL 22R alpha 2 ELISA Kit; IL-22 receptor subunit alpha-2 ELISA Kit; IL-22BP ELISA Kit; IL-22R-alpha-2 ELISA Kit; IL-22RA2 ELISA Kit; IL22 RA2 ELISA Kit; IL22BP ELISA Kit; IL22R alpha 2 ELISA Kit; Il22ra2 ELISA Kit; Interleukin 22 binding protein ELISA Kit; Interleukin 22 Receptor alpha 2 ELISA Kit; Interleukin-22 receptor subunit alpha-2 ELISA Kit; Interleukin-22-binding protein ELISA Kit; MGC150509 ELISA Kit; MGC150510 ELISA Kit; soluble 1 ELISA Kit; ZcytoR16 ELISA Kit
Abbreviation
IL22RA2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL22RA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 94    
Range % 87-98    
1:2 Average % 107    
Range % 103-112    
1:4 Average % 91    
Range % 85-97    
1:8 Average % 92    
Range % 88-96    
Recovery
The recovery of human IL22RA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 102 98-105    
EDTA plasma (n=4) 98 93-104    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
2000 1.927 2.057 1.992 1.889    
1000 1.342 1.460 1.401 1.298    
500 0.922 0.965 0.944 0.841    
250 0.651 0.663 0.657 0.554    
125 0.432 0.412 0.422 0.319    
62.5 0.254 0.269 0.262 0.159    
31.25 0.208 0.201 0.205 0.102    
0 0.104 0.102 0.103      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human IL22RA2 ELISA Kit was designed for the quantitative measurement of Human IL22RA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.; Isoform 1 may play a role in establishing and maintaining successful pregnancy.
Gene References into Functions
  1. expression higher in the epidermal compartment of the skin in comparison with dermis in both healthy skin and psoriasis skin PMID: 27676439
  2. suggest that the human IL-22BP isoforms have distinct spatial and temporal roles and coordinately fine-tune IL-22-dependent STAT3 responses in tissues as a type of rheostat. PMID: 27678220
  3. thia study shows shows that nonaffected skin of psoriasis patients displays lower expression of IL-22BP than skin of healthy controls, and that the strong IL-22 increase in lesional psoriatic skin is accompanied by a moderate induction of IL-22BP PMID: 28356382
  4. The deletion of IFNGR1 causes complete IFN-gammaR1 deficiency. Despite the deletion ending very close to the IL22RA2 gene, it does not appear to affect IL22RA2 transcription. PMID: 26931784
  5. IL-22BP is produced by eosinophils in the gut and blocks IL-22 protective actions in colitis. PMID: 26329427
  6. Through a combined approach including genetic and immunological investigation in an animal model and large-scale association studies of patients with multiple sclerosis (MS), IL-22RA2 is established as an MS risk gene. PMID: 21041731
  7. The IL-22R and IL-10R2 binding sites are juxtaposed on adjacent IL-22 surfaces contributed mostly by helices A, D, and F and loop AB. PMID: 18675824
  8. Crystallization and preliminary X-ray diffraction results of the IL-22-IL-22 binding protein (IL-22BP) complex are described. PMID: 19193995
  9. Polymorphisms in IL22RA1 are associated with severe CRS. PMID: 19393422

Show More

Hide All

Subcellular Location
Secreted.
Protein Families
Type II cytokine receptor family
Tissue Specificity
Expressed in placenta, spleen, breast, skin and lung. Also detected in intestinal tract, testis, brain, heart and thymus. No expression found in prostate, bladder, kidney, ovary, muscle, bone marrow, liver and uterus. Isoform 1 is expressed only in placen
Database Links

HGNC: 14901

OMIM: 606648

KEGG: hsa:116379

STRING: 9606.ENSP00000296980

UniGene: Hs.126891

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X