Human Interleukin 35(IL-35) ELISA Kit

Code CSB-E13126h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human IL-35 ELISA Kit was designed for the quantitative measurement of Human IL-35 protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Target Name Interleukin 35(IL-35)
Abbreviation IL-35
Species Homo sapiens (Human)
Sample Types serum, cell culture supernates, urine, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-98  
1:2 Average % 98  
Range % 94-103  
1:4 Average % 97  
Range % 90-106  
1:8 Average % 94  
Range % 88-109  
Recovery
The recovery of human IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.872 2.752 2.812 2.705  
2000 2.279 2.179 2.229 2.122  
1000 1.545 1.589 1.567 1.460  
500 0.952 1.002 0.977 0.870  
250 0.531 0.575 0.553 0.446  
125 0.341 0.366 0.354 0.247  
62.5 0.255 0.275 0.265 0.158  
0 0.108 0.105 0.107    
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-35 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled IL-35 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

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 Q&A
Q:

I am interested to know more about the IL-35 ELISA Kit (CSB-E13126h). Is the capture antibody the same as the detection antibody?

A:
Thanks for your inquiry.
IL35 is a dimer complex composed of 2 subunits EBI3 and p35.
The kit uses 2 antibodies as below, which are designed for different subunits.
Coated anti: Mouse monoclonal antibody
Detection anti: Goat polyclonal antibody Pls let me know if you have any further questions. Thank you.
Q:

I am planning to carry out a study to assess level of Interleukin from Gingival Crevicular Fluid in Chronic Periodontitis patients before and after treatment.Do you have the Interleukin 35? How many sample can be analysed?

A:
Thanks for your inquiry. We have this kit CSB-E13126h,Human Interleukin 35(IL-35) ELISA Kit to provide.This kit isapplied with sandwich enzyme immunoassay technique.For a 96T sandwich ELISA kit (doubleantibody), we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for
the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.
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