Human Interleukin 35(IL-35) ELISA Kit

Code CSB-E13126h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IL-35
Target Name Interleukin 35(IL-35)
Species Homo sapiens (Human)
Sample Types serum, cell culture supernates, urine, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-98  
1:2 Average % 98  
Range % 94-103  
1:4 Average % 97  
Range % 90-106  
1:8 Average % 94  
Range % 88-109  
Recovery
The recovery of human IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.872 2.752 2.812 2.705  
2000 2.279 2.179 2.229 2.122  
1000 1.545 1.589 1.567 1.460  
500 0.952 1.002 0.977 0.870  
250 0.531 0.575 0.553 0.446  
125 0.341 0.366 0.354 0.247  
62.5 0.255 0.275 0.265 0.158  
0 0.108 0.105 0.107    
References
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Q&A and Customer Reviews

Q&A
Q:

I am planning to carry out a study to assess level of Interleukin from Gingival Crevicular Fluid in Chronic Periodontitis patients before and after treatment.
the ELISA kit for analysis of
Interleukin 35
How many sample can be analysed by each kit ?

A:
Thanks for your inquiry.
#CSB-E13126h,Human Interleukin 35(IL-35) ELISA Kit
We have them all available.They are all appliedwith sandwich enzyme immunoassay technique.For a 96T sandwich ELISA kit (doubleantibody), we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for
the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.
If you wan to test Gingival Crevicular Fluid ,we suggest you do pre-testing first.
Q:

I am interested to know more about the IL-35 ELISA Kit (CSB-E13126h). Is the capture antibody the same as the detection antibody?

A:
Thanks for your inquiry.
IL35 is a dimer complex composed of 2 subunits EBI3 and p35.
The kit uses 2 antibodies as below, which are designed for different subunits.
Coated anti: Mouse monoclonal antibody
Detection anti: Goat polyclonal antibody Pls let me know if you have any further questions. Thank you.
Q:

Just need to know what the detection antibody is for the IL35 ELISA kit (i.e coat with p35 and detect EBI3?)

A:
Thanks for your inquiry.
CSB-E13126h
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Both antibodies are designed against 2 subunits respectively. Pls let me know if you have any further questions. Thank you.
Q:

I am planning to carry out a study to assess level of Interleukin from Gingival Crevicular Fluid in Chronic Periodontitis patients before and after treatment.Do you have the Interleukin 35? How many sample can be analysed?

A:
Thanks for your inquiry. We have this kit CSB-E13126h,Human Interleukin 35(IL-35) ELISA Kit to provide.This kit isapplied with sandwich enzyme immunoassay technique.For a 96T sandwich ELISA kit (doubleantibody), we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for
the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.
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