Human Interleukin 35(IL-35) ELISA Kit

Code CSB-E13126h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IL-35
Target Name Interleukin 35(IL-35)
Species Homo sapiens (Human)
Sample Types serum, cell culture supernates, urine, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-98  
1:2 Average % 98  
Range % 94-103  
1:4 Average % 97  
Range % 90-106  
1:8 Average % 94  
Range % 88-109  
Recovery
The recovery of human IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.872 2.752 2.812 2.705  
2000 2.279 2.179 2.229 2.122  
1000 1.545 1.589 1.567 1.460  
500 0.952 1.002 0.977 0.870  
250 0.531 0.575 0.553 0.446  
125 0.341 0.366 0.354 0.247  
62.5 0.255 0.275 0.265 0.158  
0 0.108 0.105 0.107    
References
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