Mouse Interleukin 35(IL-35) ELISA Kit

Code CSB-E13145m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IL-35
Target Name Interleukin 35(IL-35)
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %91
Range %87-97
1:2Average %95
Range %89-99
1:4Average %94
Range %90-100
1:8Average %93
Range %87-97
Recovery
The recovery of mouse IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9487-98
EDTA plasma (n=4)9691-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.461 2.391 2.426 2.235
5002.073 1.965 2.019 1.828
2501.669 1.577 1.623 1.432
1251.272 1.183 1.228 1.037
62.50.814 0.804 0.809 0.618
31.20.567 0.525 0.546 0.355
15.60.335 0.314 0.325 0.134
00.194 0.188 0.191  
References
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