Product Details

Target Name

laminin, alpha 4

Alternative Names

CMD1JJ ELISA Kit; DKFZp686D23145 ELISA Kit; LAMA3 ELISA Kit; LAMA3, formerly ELISA Kit; LAMA4 1 ELISA Kit; LAMA4 ELISA Kit; LAMA4*-1 ELISA Kit; LAMA4_HUMAN ELISA Kit; Laminin alpha 4 ELISA Kit; Laminin alpha 4 chain ELISA Kit; Laminin subunit alpha 4 precursor ELISA Kit; Laminin subunit alpha-4 ELISA Kit; Laminin-14 subunit alpha ELISA Kit; Laminin-8 subunit alpha ELISA Kit; Laminin-9 subunit alpha ELISA Kit

Abbreviation

LAMA4

Uniprot No.

Q16363

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

23.5 pg/mL-1500 pg/mL

Sensitivity

5.86 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LAMA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	95
	Range %	88-99
1:40	Average %	104
	Range %	98-108
1:80	Average %	90
	Range %	85-94
1:160	Average %	99
	Range %	90-103

Recovery

The recovery of human LAMA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-97
EDTA plasma (n=4)	101	93-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.673	2.586	2.630	2.535
750	2.180	2.145	2.163	2.068
375	1.786	1.712	1.749	1.654
187.5	1.201	1.239	1.220	1.125
94	0.653	0.648	0.651	0.556
47	0.415	0.422	0.419	0.324
23.5	0.252	0.263	0.258	0.163
0	0.093	0.097	0.095

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human LAMA4 ELISA Kit was designed for the quantitative measurement of Human LAMA4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

Code	CSB-EL012728HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human Laminin subunit alpha-4(LAMA4) ELISA kit

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