Human Liver-expressed antimicrobial peptide 2(LEAP2) ELISA kit

Instructions
Code CSB-EL012853HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name liver expressed antimicrobial peptide 2
Alternative Names LEAP2Liver-expressed antimicrobial peptide 2 ELISA Kit; LEAP-2 ELISA Kit
Abbreviation LEAP2
Uniprot No. Q969E1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LEAP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 98-111
1:2 Average % 99
Range % 90-104
1:4 Average % 97
Range % 86-103
1:8 Average % 103
Range % 95-107
Recovery
The recovery of human LEAP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 91-104
EDTA plasma (n=4) 102 95-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.512 2.600 2.556 2.403
10 2.101 1.978 2.040 1.887
5 1.403 1.415 1.409 1.256
2.5 0.824 0.844 0.834 0.681
1.25 0.489 0.501 0.495 0.342
0.625 0.311 0.323 0.317 0.164
0.312 0.231 0.226 0.229 0.076
0 0.156 0.150 0.153  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Has an antimicrobial activity.
Gene References into Functions
  1. We identified the amphibian leap2 gene which is highly related to its mammalian orthologues at both structural and sequence levels. The gene is expressed in the embryo mostly in the endoderm-derived tissues. Accordingly it is induced in pluripotent animal cap cells by FGF, activin or a combination of vegT/beta-catenin. PMID: 27335344
  2. LEAP-2 has a novel fold for a cationic antimicrobial peptide and has antibacterial activity. LEAP-2 exhibits antimicrobial activity under low-salt conditions, and there is likely to be another physiological role for the peptide. PMID: 20845358
  3. LEAP-2 displays antimicrobial activity against bacteria and yeasts. PMID: 20845358
  4. These results show that the secondary structure of human LEAP-2 has a profound impact on its antibacterial activity. PMID: 19852990
  5. Data suggest that LEAP-2 is not involved in the physiological response of GI epithelia to iron, nor is it mitogenic for epithelial cells or chemotactic for THP-1 monocytes. PMID: 20038463

Show More

Hide All

Subcellular Location Secreted
Protein Families LEAP2 family
Database Links

HGNC: 29571

OMIM: 611373

KEGG: hsa:116842

UniGene: Hs.337588

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1