Human Liver-expressed antimicrobial peptide 2(LEAP2) ELISA kit

Code CSB-EL012853HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
liver expressed antimicrobial peptide 2
Alternative Names
LEAP2Liver-expressed antimicrobial peptide 2 ELISA Kit; LEAP-2 ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
0.078 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human LEAP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 98-111
1:2 Average % 99
Range % 90-104
1:4 Average % 97
Range % 86-103
1:8 Average % 103
Range % 95-107
The recovery of human LEAP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 91-104
EDTA plasma (n=4) 102 95-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.512 2.600 2.556 2.403
10 2.101 1.978 2.040 1.887
5 1.403 1.415 1.409 1.256
2.5 0.824 0.844 0.834 0.681
1.25 0.489 0.501 0.495 0.342
0.625 0.311 0.323 0.317 0.164
0.312 0.231 0.226 0.229 0.076
0 0.156 0.150 0.153  
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human LEAP2 antibody.
  • Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial HRP-conjugated LEAP2 antibody (6 ml/bottle) ---Bind to the MSTN in the samples or standards and react with the substrate to make the solution chromogenic.
  • Substrate A (1 x 7 ml)
  • Substrate B (1 x 7 ml)
  • One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human LEAP2 ELISA Kit was designed for the quantitative measurement of Human LEAP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

(From Uniprot)
Has an antimicrobial activity.
Gene References into Functions
  1. We identified the amphibian leap2 gene which is highly related to its mammalian orthologues at both structural and sequence levels. The gene is expressed in the embryo mostly in the endoderm-derived tissues. Accordingly it is induced in pluripotent animal cap cells by FGF, activin or a combination of vegT/beta-catenin. PMID: 27335344
  2. LEAP-2 has a novel fold for a cationic antimicrobial peptide and has antibacterial activity. LEAP-2 exhibits antimicrobial activity under low-salt conditions, and there is likely to be another physiological role for the peptide. PMID: 20845358
  3. LEAP-2 displays antimicrobial activity against bacteria and yeasts. PMID: 20845358
  4. These results show that the secondary structure of human LEAP-2 has a profound impact on its antibacterial activity. PMID: 19852990
  5. Data suggest that LEAP-2 is not involved in the physiological response of GI epithelia to iron, nor is it mitogenic for epithelial cells or chemotactic for THP-1 monocytes. PMID: 20038463

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Subcellular Location
Protein Families
LEAP2 family
Database Links

HGNC: 29571

OMIM: 611373

KEGG: hsa:116842

UniGene: Hs.337588

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