Human Long palate, lung and nasal epithelium carcinoma-associated protein 4(C20orf186) ELISA kit

Instructions
Code CSB-EL003694HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name chromosome 20 open reading frame 186
Alternative Names BPI fold-containing family B member 4 ELISA Kit; Bpifb4 ELISA Kit; C20orf186 ELISA Kit; dJ726C3.5 ELISA Kit; Gm1006 ELISA Kit; Ligand-binding protein RY2G5 ELISA Kit; Long palate; lung and nasal epithelium carcinoma-associated protein 4 ELISA Kit; LPLC4_HUMAN ELISA Kit; Lplunc4 ELISA Kit
Abbreviation C20orf186
Uniprot No. P59827
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C20orf186 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %86-97
1:2Average %98
Range %92-106
1:4Average %84
Range %81-89
1:8Average %91
Range %83-98
Recovery
The recovery of human C20orf186 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10196-105
EDTA plasma (n=4)9388-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.908 3.158 3.033 2.897
8002.304 2.433 2.369 2.233
4001.478 1.475 1.477 1.341
2000.686 0.707 0.697 0.561
1000.497 0.503 0.500 0.364
500.263 0.277 0.270 0.134
250.230 0.244 0.237 0.101
00.140 0.132 0.136
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Q&A and Customer Reviews

 Customer Reviews
  • Tested application: ELISA
    Test sample: Plasma (anticoagulant)
    Species: Human
    Review: The kit was easy to do, has showen significative difference between normal and treated groups, in range with expected values.
  • Tested application: ELISA
    Test sample: Plasma
    Species: Human
    Review: We analyzed the amount of the protein in plasma of normal and frail human subjects. We have found non statistical significant trends. The test worked well, the standard curve was really good (0.99). We have to increase the statistical power by adding new sample. Internal duplicate gave similar results.

Target Data

Function May have the capacity to recognize and bind specific classes of odorants. May act as a carrier molecule, transporting odorants across the mucus layer to access receptor sites. May serve as a primary defense mechanism by recognizing and removing potentially harmful odorants or pathogenic microorganisms from the mucosa or clearing excess odorant from mucus to enable new odorant stimuli to be received (By similarity).
Gene References into Functions
  1. LAV-BPIFB4 isoform modulates eNOS signalling through Ca2+/PKC-alpha-dependent mechanism. PMID: 28419216
  2. In conclusion, BPIFB4 and CXCR4 expression classify long-lived individuals health status that correlates with maintained mononuclear cell migration. PMID: 28121621
  3. Longevity-associated variant-BPIFB4 may represent a novel therapeutic tool to fight endothelial dysfunction and promote vascular reparative processes. PMID: 26034043
Subcellular Location Secreted, Cytoplasm
Protein Families BPI/LBP/Plunc superfamily, BPI/LBP family
Tissue Specificity Expressed in nasal tissue.
Database Links

HGNC: 16179

OMIM: 615718

KEGG: hsa:149954

STRING: 9606.ENSP00000364632

UniGene: Hs.38961

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