Human MG7-Ag ELISA Kit

Code CSB-E12969h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name MG7-Ag
Abbreviation MG7-Ag
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1 U/mL-15 U/mL
Sensitivity 0.25 U/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<20%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MG7-Ag in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 83-99  
1:2 Average % 96  
Range % 91-105  
1:4 Average % 95  
Range % 87-102  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human MG7-Ag spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 93 88-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/ml OD1 OD2 Average    
15 2.048 2.027 2.038    
10 1.392 1.388 1.390    
4.5 0.728 0.731 0.730    
1.5 0.304 0.307 0.306    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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