Matrix metallopeptidase 8, also known as neutrophil collagenase, is a zinc-dependent endopeptidase that plays a key role in extracellular matrix remodeling and inflammatory processes. This enzyme is primarily stored in neutrophil granules and is released upon neutrophil activation, where it degrades various collagen types and other matrix components. MMP8 serves as an important biomarker for inflammatory conditions, tissue remodeling, and neutrophil activation. This makes it valuable for research into wound healing, periodontal disease, and various inflammatory disorders.
The Human Matrix metalloproteinase 8/Neutrophil collagenase MMP-8 ELISA kit (CSB-E04680h) is designed for quantitative measurement of MMP8 in human samples. This sandwich-based assay works with serum, plasma, and tissue homogenates with a detection range of 39 pg/mL to 2500 pg/mL and sensitivity of 9.75 pg/mL. The assay requires 50-100 μL sample volume, completes within 1-5 hours, and uses detection at 450 nm wavelength for reliable quantification of human MMP8 levels.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research examining matrix metalloproteinase activity across different biological contexts. Studies have applied the kit to quantify target levels in human serum samples from patients and in cell culture media from human airway epithelial cells. The kit supports comparative analyses between patient populations and control groups in clinical research settings.
• Inflammatory research: Measurement of matrix metalloproteinase levels alongside other inflammatory mediators including interleukins and chemokines in cell culture systems
• Clinical biomarker studies: Quantification of target protein in patient serum samples for comparative analysis between different study populations
• Respiratory research: Measurement of matrix metalloproteinase levels in bronchoalveolar lavage fluid in experimental models
• Multi-analyte profiling: Simultaneous measurement with other inflammatory markers and cytokines to characterize complex biological responses
