Human Mothers against decapentaplegic homolog 4,Smad4 ELISA Kit

Code CSB-E12749h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q13485
Lead Time 3-5 working days
Abbreviation SMAD4
Protein Biological Process 1 Transcription/Transcription regulation
Target Name SMAD family member 4
Alias DPC4, JIP, MADH4, MAD, mothers against decapentaplegic homolog 4|OTTHUMP00000163548|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|mothers against decapentaplegic homolo
Species Homo sapiens (Human)
Protein Biological Process 3 Transcription
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
HGNC 6770
RGD 3033
MGI 894293
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Smad4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %89-101
1:2Average %92
Range %88-100
1:4Average %90
Range %82-96
1:8Average %89
Range %82-103
Recovery
The recovery of human Smad4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-96
EDTA plasma (n=4)9588-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.637 2.543 2.590 2.452
8001.823 1.768 1.796 1.658
4000.957 0.935 0.946 0.808
2000.549 0.567 0.558 0.420
1000.352 0.362 0.357 0.219
500.219 0.227 0.223 0.085
250.178 0.175 0.177 0.039
00.139 0.137 0.138
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Function In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Involvement in disease Pancreatic cancer (PNCA); Juvenile polyposis syndrome (JPS); Juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome (JP/HHT); Colorectal cancer (CRC); Myhre syndrome (MYHRS)
Subcellular Location Cytoplasm, Nucleus
Protein Families Dwarfin/SMAD family
Database Links

HGNC: 6770

OMIM: 114500

KEGG: hsa:4089

STRING: 9606.ENSP00000341551

UniGene: Hs.75862

Pathway Hippo signaling pathway
TGF-beta signaling pathway
Wnt signaling pathway
Adherens junction
Cell cycle
Signaling pathways regulating pluripotency of stem cells
Th17 cell differentiation
AGE-RAGE signaling pathway in diabetic complications
Apelin signaling pathway
FoxO signaling pathway

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