Code | CSB-RA551397A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:2000 |
Creating the SMAD4 recombinant monoclonal antibody is a multistep process that involves harvesting and sequencing the SMAD4 monoclonal antibody gene, constructing aSMAD4 monoclonal antibody gene-carrying vector, transfecting it into a host cell line for culture. The synthesized peptide from human SMAD4 is used as an immunogen during SMAD4 monoclonal antibody production. The resulting SMAD4 recombinant monoclonal antibody is purified using affinity chromatography and subjected to ELISA and WB applications to determine its specificity.
SMAD4 protein is a transcription factor that plays a critical role in the TGF-beta signaling pathway. It is involved in the regulation of cell growth, differentiation, apoptosis, and tissue homeostasis. SMAD4 functions as a downstream mediator of TGF-beta signaling, translocating into the nucleus upon activation of the TGF-beta receptor complex and binding to target gene promoters to regulate their transcription. SMAD4 also interacts with other signaling pathways, including BMP, Wnt, and Notch, to regulate various cellular processes. Dysregulation of SMAD4 has been linked to the development and progression of various cancers, including pancreatic, colorectal, and gastric cancer.
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