Human Mucin-15(MUC15) ELISA kit

Code CSB-EL015218HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
mucin 15, cell surface associated
Alternative Names
Component II ELISA Kit; Glycoprotein 4 ELISA Kit; Glycoprotein C ELISA Kit; MUC 15 ELISA Kit; MUC-15 ELISA Kit; Muc15 ELISA Kit; MUC15_HUMAN ELISA Kit; Mucin 15 ELISA Kit; Mucin 15 cell surface associated ELISA Kit; Mucin-15 ELISA Kit; PAS3 ELISA Kit; PASIII ELISA Kit
Abbreviation
MUC15
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
125 pg/mL-8000 pg/mL
Sensitivity
31.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MUC15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 89-103
1:2 Average % 95
Range % 90-99
1:4 Average % 97
Range % 87-102
1:8 Average % 103
Range % 97-108
Recovery
The recovery of human MUC15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 86 81-90
EDTA plasma (n=4) 92 88-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
8000 1.972 2.027 2.000 1.863
4000 1.456 1.436 1.446 1.309
2000 0.902 0.925 0.914 0.777
1000 0.576 0.583 0.580 0.443
500 0.430 0.421 0.426 0.289
250 0.317 0.309 0.313 0.176
125 0.250 0.242 0.246 0.109
0 0.140 0.134 0.137  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human MUC15 ELISA Kit was designed for the quantitative measurement of Human MUC15 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

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Target Background

Function
(From Uniprot)
May play a role in the cell adhesion to the extracellular matrix.
Gene References into Functions
  1. Frameshift mutations of MUC15 gene is associated with gastric and colorectal cancers. PMID: 25573589
  2. Multivariate analysis suggested that MUC15 overexpression was an independent factor for prognosis (hazard risk: 3.216; P=0.009). It was concluded that MUC15 is overexpressed in glioma tissues. PMID: 24710941
  3. MUC15 inhibits dimerization of EGFR and PI3K-AKT signaling and is associated with aggressive hepatocellular carcinomas in patients. PMID: 23933603
  4. MUC15 is a strong candidate for eczema. PMID: 22657408
  5. MUC4 and MUC15 were overexpressed in papillary thyroid carcinoma, and high MUC15 expression was associated with high malignant potential. PMID: 21615302
  6. MUC15 was expressed most highly in human placenta. MUC15 mRNA & protein increased with gestational age (P < 0.05, 1st versus 3rd trimester). Differential expression of MUC15 in human placentas may play critical role in regulation of trophoblast invasion. PMID: 17720698
  7. Overexpression of MUC15 activates extracellular signal-regulated kinase 1/2 and promotes the oncogenic potential of colon cancer cells. PMID: 19520792

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Subcellular Location
[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
Tissue Specificity
Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, bone marrow, lymph node and lung.
Database Links

HGNC: 14956

OMIM: 608566

KEGG: hsa:143662

STRING: 9606.ENSP00000416753

UniGene: Hs.407152

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