Human N-Acetyl-Ser-Asp-Lys-Pro,AcSDKP ELISA Kit

Code CSB-E08949h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation AcSDKP
Target Name N-Acetyl-Ser-Asp-Lys-Pro,AcSDKP
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.235 ng/mL-15 ng/mL
Sensitivity 0.058 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human AcSDKP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %90
Range %86-94
1:2Average %100
Range %95-106
1:4Average %93
Range %87-97
1:8Average %99
Range %95-103
The recovery of human AcSDKP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10398-108
EDTA plasma (n=4)8985-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
152.465 2.346 2.406 2.246
7.52.139 2.055 2.097 1.937
3.751.812 1.746 1.779 1.619
1.881.437 1.524 1.481 1.321
0.940.962 0.971 0.967 0.807
0.470.554 0.566 0.560 0.400
0.2350.316 0.297 0.307 0.147
00.158 0.161 0.160  
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