Human NACHT, LRR and PYD domains-containing protein 1(NLRP1) ELISA kit

Code CSB-EL015864HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9C000
Lead Time 5-7 working days
Abbreviation NLRP1
Protein Biological Process 1 Apoptosis/Autophagy
Target Name NLR family, pyrin domain containing 1
Alias CARD7, CLR17.1, DEFCAP, DEFCAP-L/S, DKFZp586O1822, KIAA0926, NAC, NALP1, PP1044, SLEV1, VAMAS1, NACHT, LRR and PYD containing protein 1|NACHT, leucine rich repeat and PYD (pyrin domain) containing 1
Species Homo sapiens (Human)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.67 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.
HGNC 14374
RGD 1310963
MGI 2684861
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human NLRP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %87-99
1:2Average %88
Range %84-94
1:4Average %94
Range %88-98
1:8Average %96
Range %92-102
Recovery
The recovery of Human NLRP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9488-98
EDTA plasma (n=4)8480-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.052 2.163 2.108 2.010
6001.579 1.670 1.625 1.527
3000.984 0.978 0.981 0.883
1500.524 0.542 0.533 0.435
750.355 0.364 0.360 0.262
37.50.262 0.252 0.257 0.159
18.750.278 0.175 0.227 0.129
00.099 0.097 0.098
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Function As the sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP1, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death
Involvement in disease Vitiligo-associated multiple autoimmune disease 1 (VAMAS1); Palmoplantar carcinoma, multiple self-healing (MSPC); Autoinflammation with arthritis and dyskeratosis (AIADK)
Subcellular Location Cytoplasm, cytosol, Cytoplasm, Inflammasome, Nucleus
Protein Families NLRP family
Tissue Specificity Widely expressed (PubMed:11113115, PubMed:17164409). Abundantly expressed in primary immune cells (isoform 1 and isoform 2), including in neutrophils, monocytes/macrophages, dendritic cells (mostly Langerhans cells), and B- and T-lymphocytes (at protein l
Database Links

HGNC: 14374

OMIM: 606579

KEGG: hsa:22861

STRING: 9606.ENSP00000460475

UniGene: Hs.652273

Pathway Inflammasome Signaling
NOD-like receptor signaling pathway

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