Code | CSB-EL015864HU |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of Human NLRP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 93 | ||||
Range % | 87-99 | |||||
1:2 | Average % | 88 | ||||
Range % | 84-94 | |||||
1:4 | Average % | 94 | ||||
Range % | 88-98 | |||||
1:8 | Average % | 96 | ||||
Range % | 92-102 |
The recovery of Human NLRP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 88-98 | ||||
EDTA plasma (n=4) | 84 | 80-88 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NLRP1 (NLR family, pyrin domain containing 1) is a cytoplasmic pattern recognition receptor that plays an important role in innate immunity and inflammatory responses. As a member of the NOD-like receptor family, NLRP1 acts as an inflammasome sensor that detects pathogen-associated molecular patterns and danger-associated molecular patterns. When activated, NLRP1 forms a multiprotein inflammasome complex that triggers caspase-1 activation, leading to the processing and release of pro-inflammatory cytokines such as IL-1β and IL-18. This protein is particularly important in host defense mechanisms and has been linked to various inflammatory diseases and autoimmune conditions.
The Human NACHT, LRR and PYD domains-containing protein 1 (NLRP1) ELISA kit (CSB-EL015864HU) is designed for quantitative measurement of NLRP1 in human samples. This sandwich ELISA works with serum, plasma, tissue homogenates, and cell lysates with a detection range of 18.75 pg/mL to 1200 pg/mL and sensitivity of 4.67 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for NLRP1 quantification in various biological specimens.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in clinical research to measure NLRP1 protein levels in human serum samples from patients presenting with cardiovascular symptoms. The kit supports research into inflammasome-related pathways and their potential connections with coronary artery disease severity and clinical presentations.
• Cardiovascular research: Measuring NLRP1 levels in patients with unstable angina and coronary artery disease to explore potential biomarker relationships with disease severity and coronary lesion characteristics
• Inflammasome studies: Studying pyroptosis-related protein expression patterns, including NLRP1 as part of broader inflammatory pathway research alongside other pyroptosis regulators
• Clinical biomarker analysis: Examining serum NLRP1 concentrations in patient populations to study correlations with cardiovascular risk factors and disease progression markers
• Acute care research: Measuring NLRP1 levels in patients experiencing acute chest pain symptoms within clinical hospital settings for potential diagnostic or prognostic applications
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