Human Neural -Cadherin, N-Cad ELISA kit

Instructions
Code CSB-E09718h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cadherin 2, type 1, N-cadherin (neuronal)
Alternative Names CADH2_HUMAN ELISA Kit; Cadherin 2 ELISA Kit; Cadherin 2 N cadherin neuronal ELISA Kit; Cadherin 2 type 1 ELISA Kit; Cadherin 2 type 1 N cadherin neuronal ELISA Kit; Cadherin 2; type 1; N-cadherin (neuronal) ELISA Kit; Cadherin-2 ELISA Kit; Cadherin2 ELISA Kit; Calcium dependent adhesion protein neuronal ELISA Kit; CD325 ELISA Kit; CD325 antigen ELISA Kit; CDH2 ELISA Kit; CDHN ELISA Kit; CDw325 ELISA Kit; CDw325 antigen ELISA Kit; N cadherin 1 ELISA Kit; N-cadherin ELISA Kit; NCAD ELISA Kit; Neural cadherin ELISA Kit; OTTHUMP00000066304 ELISA Kit; OTTHUMP00000067378 ELISA Kit
Abbreviation CDH2
Uniprot No. P19022
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human N-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %80-100
1:2Average %97
Range %91-104
1:4Average %97
Range %92-108
1:8Average %94
Range %85-98
Recovery
The recovery of human N-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-105
EDTA plasma (n=4)9790-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
4002.152 2.174 2.163 2.005
2001.832 1.824 1.828 1.670
1001.220 1.251 1.236 1.078
500.926 0.974 0.950 0.792
250.574 0.543 0.559 0.401
12.50.406 0.426 0.416 0.258
6.250.335 0.312 0.324 0.166
00.152 0.164 0.158  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Can #CSB-E09718h ELISA Kit be tested with a cell culture medium?

A:
Thanks for your inquiry. Usually we suggest you test the sample types mentioned in the kit manual. You can do a pretest if you want to test cell culture medium.

Target Data

Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone
Gene References into Functions
  1. Human keratinocyte cells reduce the N-cadherin levels of co-cultured melanoma cells. Cell-to-cell contact is necessary for this keratinocyte-mediated N-cadherin reduction. Keratinocytes reduce intracellular calcium in co-cultured melanoma cells. PMID: 29902459
  2. The FGFR4-388arg variant promotes lung cancer progression by N-cadherin induction. PMID: 29402970
  3. This study showed that the significant N-cadherin expression especially in high-grade meningioma group. PMID: 29297710
  4. Our experiments presented a new mechanism adopted by GDNF supporting glioma development and indicated a possible therapeutic potential via the inhibition of proN-cadherin/FGFR1 interaction. PMID: 29750313
  5. In patients with gastric cancer, the strong expression of N-cadherin in lymph nodes correlated with more lymph nodes metastasis, an advanced stage, and poor prognosis PMID: 28247164
  6. Regulatory networks specifying cortical interneurons from human embryonic stem cells reveal roles for CHD2 in interneuron development PMID: 29229852
  7. A study with nonsyndromic cleft lip with or without cleft palate (NSCL+/-P) cases (N=292) and controls (N=287) established association of a SNP in intron 2 of CDH2 with NSCL+/-P as a risk factor. PMID: 29524576
  8. Results show that N-cadherin is highly expressed in 70% of patients with glioma. However, N-cadherin, as a representative EMT marker, have limited prognostic value in glioma. Nonetheless, the EMT process in gliomas may be compounded by enhanced N-cadherin expression supported by unfavorable prognostic outcomes. PMID: 28851312
  9. Findings reveal the importance of CDH2-mediated cell contacts in preserving features of the juvenile nucleus pulposus cell phenotype. PMID: 27292569
  10. Tumor-promoting role of N-cadherin in thyroid cancer was closely related to the activities of the MAPK/Erk, the phosphatidylinositol-3-kinase (PI3K)/Akt and p16/Rb signaling pathways. PMID: 28042956
  11. Triple negative breast cancer cells surviving short-term chemotherapy treatment are more invasive than bulk tumor cells. Cell surface pro-N-cadherin expression is associated with the invasive and chemo-resistant behaviors of this tumor cell subset. PMID: 27768598
  12. Sec8 regulates N-cadherin expression by controlling Smad3 and Smad4 expression through CBP, thereby mediating the epithelial-mesenchymal transition. PMID: 27769780
  13. Results found that the N-glycan at N402 is comprised of beta 1,6 GlcNAc branching and that in glioma, deficient N402 N-glycosylation destabilises N-cadherin and leads to its proteasomal degradation. Destabilisation of N-cadherin inhibits cadherin-mediated cell-cell adhesion and promotes cell migration. Our findings imply that the control of N-cadherin stability by N-glycosylation is important in glioma migration. PMID: 27864899
  14. In adrenocortical carcinomas, the loss of N-cadherin is a frequent phenomenon while the existence of TERT promoter mutations is not, and nuclear telomerase expression is present in only a minority of cases. PMID: 27886397
  15. High CDH2 expression is associated with M2-type acute myeloid leukemia. PMID: 27064800
  16. Migration of bone marrow-mesenchymal stem cells in response to TGF-beta was mediated through N-cadherin and noncanonical TGF-beta signals. PMID: 28213973
  17. Studied the roles of MIRN145 in lung adenocarcinoma (LAC) and its targeting of N-cadherin. Knockdown of N-cadherin inhibited invasion and migration of LAC cell lines similar to overexpression of MIRN45. PMID: 28120164
  18. Genetic mutations in CDH2-encoded N-cadherin may represent a novel pathogenetic basis for arrhythmogenic cardiomyopathy. PMID: 28326674
  19. Extracellular and intracellular cleavage of N-cadherin might be involved in elevated MMP-9 expression enhancing tumor cell invasion. PMID: 27737648
  20. a mechanism where the membrane organization of CD82, through specific posttranslational modifications, regulates N-cadherin clustering and membrane density, which impacts the in vivo trafficking of AML cells. PMID: 26592446
  21. fluorescent imaging to demonstrate Ncad-mediated single cell responses to developmental cues within hydrogels towards chondrogenesis. PMID: 27106637
  22. Our data demonstrate that N-Cadherin was markedly overexpressed and miR-199b-5p was significantly downregulated in hepatocellular carcinoma (HCC). MiR-199b-5p exerts inhibitory effects on EMT, and directly targets N-cadherin in HCC, supporting the potential utility of miR-199b-5p as a promising strategy to treat HCC PMID: 28588321
  23. Study demonstrates a critical role of presynaptic cadherin/catenin/p140Cap cell adhesion complexes in stabilizing functional synapses and spines in the developing neocortex. PMID: 28641114
  24. investigated the in vitro tumor/metastasis suppressor effects of plakoglobin in ovarian cancer cell lines with mutant p53 expression and different cadherin profiles PMID: 27144941
  25. These results uncover a new role for p120 catenin bound to the N-cadherin precursor ensuring its trafficking through the biosynthetic pathway towards the cell surface. PMID: 27254316
  26. Finding that the cells expressing N-cadherin gave rise to tumors with no expression of N-cadherin is in agreement with the classical view of epithelial to mesenchymal transition. Epithelial to mesenchymal transition and N-cadherin are associated with dissemination and not with the ability to establish new tumor growth. PMID: 27224422
  27. These data implicate CDH2 mutations as novel genetic causes of Arrhythmogenic Right Ventricular Cardiomyopathy and contribute to a more complete identification of disease genes involved in cardiomyopathy. PMID: 28280076
  28. High expression of N-cadherin is associated with bladder cancer. PMID: 27683053
  29. Snail and N-cadherin are constitutively and inducibly expressed in papillary thyroid carcinoma PMID: 26219900
  30. SFMSCs increased through upregulation of the activated lymphocyte cell adhesion molecule (ALCAM) and N-cadherin by microRNA-192 and -218 downregulation, similar to BMMSCs and ADMSCs. PMID: 28039611
  31. the synergy between N-cadherin and FGFR signalling that ensure cellular reorganization during cell movements, mainly during cancer cell migration and metastasis but also during developmental processes. PMID: 27320194
  32. Underexpression of CDH2 is associated with adrenocortical tumors. PMID: 27468715
  33. Of the seven polymorphisms, two reached statistical significance for obsessive-compulsive disorder under additive and codominant models of inheritance PMID: 26093892
  34. Targeting N-cadherin may be a promising therapeutic approach, particularly in cisplatin-resistant, therapy refractory and metastatic Germ cell tumor. PMID: 26451610
  35. Metformin's anti-cancer therapeutic effect is mediated through different molecular mechanism in wild-type vs. deficient N-cadherin cancer cells. PMID: 26359363
  36. Expression data of NCAD shows no association with advanced gastric cancer brain metastasis. PMID: 26260219
  37. CDH2 was found to be a susceptibility gene for Gilles de la Tourette syndrome in a Danish cohort. PMID: 26032459
  38. Existing knowledge regarding the role of CDH2 and CDH11 during development and differentiation in vivo and in vitro is reviewed. [review] PMID: 25771201
  39. N-cadherin was widely expressed in CRC cell lines and silencing of N-cadherin suppressed the proliferation and migration of the CRC cell line HT-29 by upregulating E-cadherin, suggesting a potential role of N-cadherin in inducing EMT. PMID: 25936636
  40. N-cadherin and connexin 43 expression in in group of diffuse astrocytomas and anaplastic astrocytomas may be evidence for their role in tumor formation and progression PMID: 25386667
  41. Foxn3 is a direct transcriptional suppressor of N-cadherin in colorectal metastasis tissues. PMID: 26069251
  42. the expression of N-cadherin was associated with Vasculogenic mimicry formation in esophageal squamous cell carcinoma PMID: 25575439
  43. Results suggest that N-cadherin may promote motility and invasiveness through distinct mechanisms and that beta-catenin may be an integral mediator of N-cadherin-dependent invasive signaling in oral epithelia. PMID: 25175499
  44. Results demonstrate that miR-194 affected the growth and metastasis of osteosarcoma cells both in vitro and in vivo suggesting that miR-194 functions as tumor suppressor gene probably by downregulating CDH2 and IGF1R. PMID: 25096247
  45. association of beta-catenin with N-cadherin is regulated by actin polymerization during contractile activation PMID: 25713069
  46. Decreased N-cadherin expression is linked to increased ADAM-10 expression in atherosclerotic lesions. PMID: 24985126
  47. This indicates that similar biofunctionalization approaches based on N-cadherin and L1 can be translated to 3-D "transplantable" scaffolds with enhanced neurotrophic behaviors. PMID: 24914828
  48. miR-199a targeted the sequence within the 3'UTR of the N-cadherin mRNA and suppressed the TGF-beta1-induced increase in the protein level of N-cadherin in a manner independent of SNAI1. PMID: 25041364
  49. N-cadherin and CD133 expressions are strongly correlated and N-cadherin appears as a potential breast cancer metastases marker in a specific patient subpopulation. PMID: 24962344
  50. High N-cadherin expression is associated with malignant bone and soft tissue tumors. PMID: 23799912

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Cell membrane, sarcolemma, Cell junction, Cell surface
Database Links

HGNC: 1759

OMIM: 114020

KEGG: hsa:1000

STRING: 9606.ENSP00000269141

UniGene: Hs.464829

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