Code | CSB-RA243509A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The preparation of the CDH2 recombinant monoclonal antibody is based on protein technology and DNA recombinant technology. First, mice were immunized with a synthesized peptide derived from human N Cadherin. After a certain period of time, the spleen of the mice was removed under aseptic conditions. The total RNA of spleen cells was extracted, and the cDNA synthesized by RNA reverse transcription was used as the template for PCR amplification of the CDH2 antibody gene. The obtained CDH2 antibody gene is introduced into a vector, which is then transfected into host cells for culture. The CDH2 recombinant monoclonal antibody is purified from the cell culture supernatant by affinity chromatography. It has been rigorously verified and can be used for human CDH2 protein detection in ELISA and IHC experiments.
The CDH2 protein, also known as N-cadherin, is involved in various cellular processes, including cell adhesion, migration, signaling, tissue development, maintenance, and repair. In cells, the CDH2 protein forms homophilic interactions with other CDH2 proteins in neighboring cells, leading to the formation of cell-cell adhesions. During development and tissue repair, CDH2 promotes cell migration by binding to extracellular matrix proteins, such as fibronectin and laminin, as well as by interacting with intracellular signaling molecules, such as the small GTPase RhoA. Dysregulation of its expression or function can lead to various diseases, including cancer and cardiovascular disease.
Applications : Western blot
Sample type: cells
Review: Western blot analysis of apoptosis- and EMT-related molecules in LM6 cells with BAZ2A knockdown.
By Anonymous