Human Neuroblastoma suppressor of tumorigenicity 1(NBL1) ELISA kit

Code CSB-EL015485HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
neuroblastoma, suppression of tumorigenicity 1
Alternative Names
D1S1733E ELISA Kit; DAN ELISA Kit; DAN domain family member 1 ELISA Kit; DAND 1 ELISA Kit; DAND1 ELISA Kit; Differential screening selected gene aberrant in neuroblastoma ELISA Kit; MGC8972 ELISA Kit; NB ELISA Kit; NBL 1 ELISA Kit; NBL1 ELISA Kit; NBL1_HUMAN ELISA Kit; Neuroblastoma candidate region suppression of tumorigenicity 1 ELISA Kit; Neuroblastoma suppression of tumorigenicity 1 ELISA Kit; Neuroblastoma suppressor of tumorigenicity 1 ELISA Kit; NO 3 ELISA Kit; NO3 ELISA Kit; Protein N03 ELISA Kit; Zinc finger protein DAN ELISA Kit
Abbreviation
NBL1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
34.4 pg/mL-2200 pg/mL
Sensitivity
8.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NBL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 84-96
1:2 Average % 97
Range % 91-105
1:4 Average % 103
Range % 97-107
1:8 Average % 89
Range % 83-94
Recovery
The recovery of human NBL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 100 94-105
EDTA plasma (n=4) 106 101-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2200 1.755 1.821 1.788 1.665
1100 1.285 1.289 1.287 1.164
550 0.848 0.845 0.847 0.724
275 0.547 0.533 0.540 0.417
137.5 0.353 0.365 0.359 0.236
68.75 0.298 0.321 0.310 0.187
34.4 0.225 0.214 0.220 0.097
0 0.125 0.121 0.123  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human NBL1 ELISA Kit was designed for the quantitative measurement of Human NBL1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 34.4 pg/mL-2200 pg/mL and the sensitivity is 8.6 pg/mL.

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Target Background

Function
(From Uniprot)
Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
Subcellular Location
Secreted.
Protein Families
DAN family
Tissue Specificity
Most abundant in normal lung and meningioma.
Database Links

HGNC: 7650

OMIM: 600613

KEGG: hsa:100532736

STRING: 9606.ENSP00000289749

UniGene: Hs.466662

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