Human POTE ankyrin domain family member E(POTEE) ELISA kit

Code CSB-EL740863HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
POTE ankyrin domain family member E
Alternative Names
POTEE ELISA Kit; A26C1A ELISA Kit; POTE2 ELISA Kit; POTE ankyrin domain family member E ELISA Kit; ANKRD26-like family C member 1A ELISA Kit; Prostate ELISA Kit; ovary ELISA Kit; testis-expressed protein on chromosome 2 ELISA Kit; POTE-2 ELISA Kit
Abbreviation
POTEE
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human POTEE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 89-97
1:2 Average % 98
Range % 93-103
1:4 Average % 102
Range % 98-106
1:8 Average % 91
Range % 86-97
Recovery
The recovery of human POTEE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 96-108
EDTA plasma (n=4) 89 83-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.786 2.934 2.860 2.729
800 2.214 2.341 2.278 2.147
400 1.425 1.467 1.446 1.315
200 0.743 0.754 0.749 0.618
100 0.456 0.446 0.451 0.320
50 0.327 0.312 0.320 0.189
25 0.216 0.211 0.214 0.083
0 0.132 0.130 0.131  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human POTEE ELISA Kit was designed for the quantitative measurement of Human POTEE protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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