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Human Pancreatic Amylase,PAMY ELISA Kit

Human Pancreatic Amylase,PAMY ELISA Kit

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Code	CSB-E09691h
Size	96T,5×96T,10×96T
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Target Data

Product Details

Target Name

amylase, alpha 2A (pancreatic)

Alternative Names

AMY2A ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit

Abbreviation

AMY2A

Uniprot No.

P04746

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

47 mU/mL-3000 mU/mL

Sensitivity

11.75 mU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PAMY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-99
1:2	Average %	102
	Range %	96-107
1:4	Average %	94
	Range %	87-98
1:8	Average %	86
	Range %	81-92

Recovery

The recovery of human PAMY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	91-103
EDTA plasma (n=4)	102	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
3000	2.066	2.154	2.110	1.992
1500	1.496	1.589	1.543	1.425
750	1.007	1.043	1.025	0.907
375	0.634	0.647	0.641	0.523
187.5	0.375	0.382	0.379	0.261
93.75	0.267	0.273	0.270	0.152
46.88	0.186	0.189	0.188	0.070
0	0.117	0.118	0.118

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Data

Gene References into Functions	Data show that serum amylase was found to be correlated with waist circumference, ghrelin and PYY3-36. PMID: 26495778 Data show that amylase-alpha2A autoantibodies may help to diagnosis of autoimmune pancreatitis (AIP) and to differentiate AIP subtypes. PMID: 26335011 Studies indicate that chloride activates alpha-amylases and ACE, and gaining insight into the potential mechanisms by which chloride functions in PSII. PMID: 21366335 Mammalian pancreatic alpha-amylases share a common carbohydrate binding activity and specifically bind to the intestinal brush border. PMID: 22584580 We demonstrated that AMY2A is frequently silenced in gastric carcinoma deletion 1p21.1. PMID: 20428766 A new approach for the discovery and subsequent structural elucidation of oligosaccharide-based inhibitors of alpha-amylases based upon autoglucosylation of known alpha-glucosidase inhibitors is presented. PMID: 15304511 Autoantibody against amylase alpha-2A is a novel diagnostic marker for both autoimmune pancreatitis and fulminant type 1 diabetes. PMID: 19001184 Show More Hide All
Subcellular Location	Secreted, extracellular space
Protein Families	Glycosyl hydrolase 13 family
Tissue Specificity	Detected in pancreas (at protein level).
Database Links	HGNC: 477 OMIM: 104650 KEGG: hsa:279 STRING: 9606.ENSP00000397582 UniGene: Hs.654437

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Human Pancreatic Amylase,PAMY ELISA Kit

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