Human Pancreatic Amylase,PAMY ELISA Kit

Instructions
Code CSB-E09691h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name amylase, alpha 2A (pancreatic)
Alternative Names AMY2A ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
Abbreviation AMY2A
Uniprot No. P04746
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 47 mU/mL-3000 mU/mL
Sensitivity 11.75 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PAMY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 95    
Range % 89-99    
1:2 Average % 102    
Range % 96-107    
1:4 Average % 94    
Range % 87-98    
1:8 Average % 86    
Range % 81-92    
Recovery
The recovery of human PAMY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 98 91-103    
EDTA plasma (n=4) 102 96-105    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
mU/ml OD1 OD2 Average Corrected    
3000 2.066 2.154 2.110 1.992    
1500 1.496 1.589 1.543 1.425    
750 1.007 1.043 1.025 0.907    
375 0.634 0.647 0.641 0.523    
187.5 0.375 0.382 0.379 0.261    
93.75 0.267 0.273 0.270 0.152    
46.88 0.186 0.189 0.188 0.070    
0 0.117 0.118 0.118      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Gene References into Functions
  1. Data show that serum amylase was found to be correlated with waist circumference, ghrelin and PYY3-36. PMID: 26495778
  2. Data show that amylase-alpha2A autoantibodies may help to diagnosis of autoimmune pancreatitis (AIP) and to differentiate AIP subtypes. PMID: 26335011
  3. Studies indicate that chloride activates alpha-amylases and ACE, and gaining insight into the potential mechanisms by which chloride functions in PSII. PMID: 21366335
  4. Mammalian pancreatic alpha-amylases share a common carbohydrate binding activity and specifically bind to the intestinal brush border. PMID: 22584580
  5. We demonstrated that AMY2A is frequently silenced in gastric carcinoma deletion 1p21.1. PMID: 20428766
  6. A new approach for the discovery and subsequent structural elucidation of oligosaccharide-based inhibitors of alpha-amylases based upon autoglucosylation of known alpha-glucosidase inhibitors is presented. PMID: 15304511
  7. Autoantibody against amylase alpha-2A is a novel diagnostic marker for both autoimmune pancreatitis and fulminant type 1 diabetes. PMID: 19001184

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Subcellular Location Secreted, extracellular space
Protein Families Glycosyl hydrolase 13 family
Tissue Specificity Detected in pancreas (at protein level).
Database Links

HGNC: 477

OMIM: 104650

KEGG: hsa:279

STRING: 9606.ENSP00000397582

UniGene: Hs.654437

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