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Human Pepsinogen II ELISA Kit

Citations (2) Protocol MSDS
Code CSB-E17539h
Size 96T,5×96T,10×96T
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Product Details

Target Name
Pepsinogen II
Abbreviation
PG II
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates
Detection Range
6.25 ng/mL-400 ng/mL
Sensitivity
1.56 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %102
Range %96-107
1:2Average %94
Range %90-98
1:4Average %95
Range %89-99
1:8Average %93
Range %87-98
Recovery
The recovery of human pepsinogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-103
EDTA plasma (n=4)8681-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
4002.624 2.733 2.679 2.505
2002.189 2.077 2.133 1.959
1001.425 1.353 1.389 1.215
500.746 0.735 0.741 0.567
250.437 0.425 0.431 0.257
12.50.312 0.326 0.319 0.145
6.250.238 0.234 0.236 0.062
00.175 0.173 0.174
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Human PG II ELISA Kit was designed for the quantitative measurement of Human PG II protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

Citations

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