Human Peptidyl-prolyl cis-trans isomerase C(PPIC) ELISA kit

Code CSB-EL018473HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
peptidylprolyl isomerase C (cyclophilin C)
Alternative Names
Cyclophilin C ELISA Kit; CYPC ELISA Kit; MGC3673 ELISA Kit; Parvulin ELISA Kit; Peptidyl prolyl cis trans isomerase C ELISA Kit; Peptidyl-prolyl cis-trans isomerase C ELISA Kit; Peptidylprolyl isomerase C (cyclophilin C) ELISA Kit; peptidylprolyl isomerase C ELISA Kit; PPIase C ELISA Kit; ppiC ELISA Kit; PPIC_HUMAN ELISA Kit; Rotamase C ELISA Kit
Abbreviation
PPIC
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PPIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 92-104
1:2 Average % 91
Range % 82-98
1:4 Average % 103
Range % 98-108
1:8 Average % 87
Range % 82-98
Recovery
The recovery of human PPIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 85-98
EDTA plasma (n=4) 111 105-115
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.651 2.656 2.654 2.497
750 2.322 2.251 2.287 2.130
375 1.645 1.655 1.650 1.493
187.5 1.002 0.974 0.988 0.831
94 0.587 0.544 0.566 0.409
47 0.389 0.401 0.395 0.238
23.5 0.262 0.245 0.254 0.097
0 0.156 0.157 0.157  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PPIC ELISA Kit was designed for the quantitative measurement of Human PPIC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

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Target Background

Function
(From Uniprot)
PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
Gene References into Functions
  1. Independent validation with glioma patients tissue (n = 70) and normal brain tissue (n = 19) found PPIC, EMP3 and CHI3L1 were up-regulated in glioma tissue. Survival value validation showed that the three genes correlated with patient survival by Kaplan-Meir analysis, including grades, age and therapy. PMID: 27801851
  2. Cyclophilin C participates in cytomegalovirus US2 protein-mediated degradation of major histocompatibility complex class I molecules. PMID: 26691022
Subcellular Location
Cytoplasm.
Protein Families
Cyclophilin-type PPIase family
Tissue Specificity
Expressed in kidney, skeletal muscle, pancreas, heart, lung, liver and to a lower extent in brain.
Database Links

HGNC: 9256

OMIM: 123842

KEGG: hsa:5480

STRING: 9606.ENSP00000303057

UniGene: Hs.110364

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