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| Code | CSB-E08110h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:10 | Average % | 104 | ||||
| Range % | 100-109 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:40 | Average % | 85 | ||||
| Range % | 81-92 | |||||
| The recovery of human PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 97 | 93-101 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human phospholipidase A2 (PLA2) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PLA2 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PLA2 bound in the initial step.
PLA2 plays key roles in multiple cellular responses, including phospholipid digestion & metabolism, host defense, and signal transduction. PLA2 catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. PLA2 is a key enzyme in the regulation of lipid mediators of the inflammatory process. Evidence has shown that PLA2 is involved in all stages of pathogen encounter, from the initial signaling to the uptake, degradation, and presentation of the antigen.
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