Human Phospholipidase A2,PLA2 ELISA Kit

Code CSB-E08110h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name phospholipase A2, group IB (pancreas)
Alternative Names Group IB phospholipase A2 ELISA Kit; Group IB secretory phospholipase A2 ELISA Kit; MGC119834 ELISA Kit; MGC119835 ELISA Kit; PA21B_HUMAN ELISA Kit; Phosphatidylcholine 2 acylhydrolase ELISA Kit; Phosphatidylcholine 2-acylhydrolase 1B ELISA Kit; Phospholipase A2 ELISA Kit; Phospholipase A2 group IB (pancreas) ELISA Kit; Phospholipase A2 group IB ELISA Kit; PLA 2 ELISA Kit; PLA2 ELISA Kit; PLA2A ELISA Kit; PLA2G1B ELISA Kit; PPLA 2 ELISA Kit; PPLA2 ELISA Kit; sPLA2IB ELISA Kit
Abbreviation PLA2G1B
Uniprot No. P04054
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.45 ng/mL-30 ng/mL
Sensitivity 0.11 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %93
Range %88-97
1:10Average %104
Range %100-109
1:20Average %95
Range %91-99
1:40Average %85
Range %81-92
Recovery
The recovery of human PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-96
EDTA plasma (n=4)9793-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
302.534 2.452 2.493 2.384
151.652 1.576 1.614 1.505
7.51.066 1.004 1.035 0.926
3.750.578 0.561 0.570 0.461
1.80.387 0.401 0.394 0.285
0.90.238 0.245 0.242 0.133
0.450.179 0.187 0.183 0.074
00.105 0.112 0.109  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Secretory calcium-dependent phospholipase A2 that primarily targets dietary phospholipids in the intestinal tract. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation in the intestinal tract. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. May act in an autocrine and paracrine manner. Upon binding to the PLA2R1 receptor can regulate podocyte survival and glomerular homeostasis. Has anti-helminth activity in a process regulated by gut microbiota. Upon helminth infection of intestinal epithelia, directly affects phosphatidylethanolamine contents in the membrane of helminth larvae, likely controlling an array of phospholipid-mediated cellular processes such as membrane fusion and cell division while providing for better immune recognition, ultimately reducing larvae integrity and infectivity.
Gene References into Functions
  1. sPLA2-IB was correlated with the level of proteinuria in membranous nephropathy patients suggesting to be a potential biomarker for monitoring disease severity and therapeutic effects of both primary membranous nephropathy and secondary membranous nephropathy. PMID: 29649452
  2. The sPLA2 IB-PLA2R interaction stimulated podocyte apoptosis through activating ERK1/2 and cPLA2alpha and through increasing the podocyte AA content PMID: 25335547
  3. TNF-alpha-induced cPLA2 expression and PGE2 release were mediated through a Jak2/PDGFR/PI3K/Akt/p42/p44 MAPK/Elk-1 pathway in human lung epithelial cells. PMID: 24441870
  4. Exogenously added sPLA(2)-IB decreases phagocytosis of photoreceptor outer segments regardless of enzymatic activity. PMID: 22680611
  5. stimulation of three isoforms of PLA2 by thapsigargin liberates free AA that, in turn, induces capacitative calcium influx in human T-cells PMID: 12423354
  6. results indicate a selective sorting of a cell-derived cPLA2 during human cytomegalovirus maturation, which is further required for infectivity PMID: 15220446
  7. Group IB phospholipase A2 (PLA2G1B) stimulates leukotriene B4 (LTB4) production in the absence of cytochalasin B in human neutrophils. PMID: 16005851
  8. Here, we report sPLA2-IB in rat and human brain as well as in neurons in primary culture. The distribution of sPLA2-IB seems to be mainly neuronal, with the highest abundance occurring in the cerebral cortex and hippocampus. PMID: 16392040
  9. a critical regulatory role of arachidonate reacylation that limits leukotriene biosynthesis in concert with 5-lipoxygenase and cytosolic phospholipase A(2)alpha activation PMID: 16495221
  10. Results describe the structural basis for bile salt inhibition of pancreatic phospholipase A2. PMID: 17434532
  11. MMP-2/9 production is regulated by sPLA2-IB acting as a receptor ligand to activate cPLA2 PMID: 17981679
  12. pro-hG1B forms a trimer and PROP occupies the catalytic cavity and can be self-cleaved at 37 degrees C; A new membrane-bound surface and activation mechanism are proposed based on the trimeric model of pro-hG1B PMID: 19297324

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Subcellular Location Secreted. Note=Secreted from pancreatic acinar cells in its inactive form.
Protein Families Phospholipase A2 family
Tissue Specificity Selectively expressed in pancreas, lung, liver and kidney. Also detected at lower levels in ovary and testis.
Database Links

HGNC: 9030

OMIM: 172410

KEGG: hsa:5319

STRING: 9606.ENSP00000312286

UniGene: Hs.992

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