Human Phospholipidase A2,PLA2 ELISA Kit

Product Details

Citations

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

phospholipase A2, group IB (pancreas)

Alternative Names

Group IB phospholipase A2 ELISA Kit; Group IB secretory phospholipase A2 ELISA Kit; MGC119834 ELISA Kit; MGC119835 ELISA Kit; PA21B_HUMAN ELISA Kit; Phosphatidylcholine 2 acylhydrolase ELISA Kit; Phosphatidylcholine 2-acylhydrolase 1B ELISA Kit; Phospholipase A2 ELISA Kit; Phospholipase A2 group IB (pancreas) ELISA Kit; Phospholipase A2 group IB ELISA Kit; PLA 2 ELISA Kit; PLA2 ELISA Kit; PLA2A ELISA Kit; PLA2G1B ELISA Kit; PPLA 2 ELISA Kit; PPLA2 ELISA Kit; sPLA2IB ELISA Kit

Abbreviation

PLA2G1B

Uniprot No.

P04054

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.45 ng/mL-30 ng/mL

Sensitivity

0.11 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	93
	Range %	88-97
1:10	Average %	104
	Range %	100-109
1:20	Average %	95
	Range %	91-99
1:40	Average %	85
	Range %	81-92

Recovery

The recovery of human PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	93-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.534	2.452	2.493	2.384
15	1.652	1.576	1.614	1.505
7.5	1.066	1.004	1.035	0.926
3.75	0.578	0.561	0.570	0.461
1.8	0.387	0.401	0.394	0.285
0.9	0.238	0.245	0.242	0.133
0.45	0.179	0.187	0.183	0.074
0	0.105	0.112	0.109

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This human phospholipidase A2 (PLA2) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human PLA2 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PLA2 bound in the initial step.

PLA2 plays key roles in multiple cellular responses, including phospholipid digestion & metabolism, host defense, and signal transduction. PLA2 catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. PLA2 is a key enzyme in the regulation of lipid mediators of the inflammatory process. Evidence has shown that PLA2 is involved in all stages of pathogen encounter, from the initial signaling to the uptake, degradation, and presentation of the antigen.

Target Background

Function
(From Uniprot)

Secretory calcium-dependent phospholipase A2 that primarily targets dietary phospholipids in the intestinal tract. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation in the intestinal tract. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. May act in an autocrine and paracrine manner. Upon binding to the PLA2R1 receptor can regulate podocyte survival and glomerular homeostasis. Has anti-helminth activity in a process regulated by gut microbiota. Upon helminth infection of intestinal epithelia, directly affects phosphatidylethanolamine contents in the membrane of helminth larvae, likely controlling an array of phospholipid-mediated cellular processes such as membrane fusion and cell division while providing for better immune recognition, ultimately reducing larvae integrity and infectivity.

Gene References into Functions

sPLA2-IB was correlated with the level of proteinuria in membranous nephropathy patients suggesting to be a potential biomarker for monitoring disease severity and therapeutic effects of both primary membranous nephropathy and secondary membranous nephropathy. PMID: 29649452
The sPLA2 IB-PLA2R interaction stimulated podocyte apoptosis through activating ERK1/2 and cPLA2alpha and through increasing the podocyte AA content PMID: 25335547
TNF-alpha-induced cPLA2 expression and PGE2 release were mediated through a Jak2/PDGFR/PI3K/Akt/p42/p44 MAPK/Elk-1 pathway in human lung epithelial cells. PMID: 24441870
Exogenously added sPLA(2)-IB decreases phagocytosis of photoreceptor outer segments regardless of enzymatic activity. PMID: 22680611
stimulation of three isoforms of PLA2 by thapsigargin liberates free AA that, in turn, induces capacitative calcium influx in human T-cells PMID: 12423354
results indicate a selective sorting of a cell-derived cPLA2 during human cytomegalovirus maturation, which is further required for infectivity PMID: 15220446
Group IB phospholipase A2 (PLA2G1B) stimulates leukotriene B4 (LTB4) production in the absence of cytochalasin B in human neutrophils. PMID: 16005851
Here, we report sPLA2-IB in rat and human brain as well as in neurons in primary culture. The distribution of sPLA2-IB seems to be mainly neuronal, with the highest abundance occurring in the cerebral cortex and hippocampus. PMID: 16392040
a critical regulatory role of arachidonate reacylation that limits leukotriene biosynthesis in concert with 5-lipoxygenase and cytosolic phospholipase A(2)alpha activation PMID: 16495221
Results describe the structural basis for bile salt inhibition of pancreatic phospholipase A2. PMID: 17434532
MMP-2/9 production is regulated by sPLA2-IB acting as a receptor ligand to activate cPLA2 PMID: 17981679
pro-hG1B forms a trimer and PROP occupies the catalytic cavity and can be self-cleaved at 37 degrees C; A new membrane-bound surface and activation mechanism are proposed based on the trimeric model of pro-hG1B PMID: 19297324

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Subcellular Location

Secreted. Note=Secreted from pancreatic acinar cells in its inactive form.

Protein Families

Phospholipase A2 family

Tissue Specificity

Selectively expressed in pancreas, lung, liver and kidney. Also detected at lower levels in ovary and testis.

Database Links

HGNC: 9030

OMIM: 172410

KEGG: hsa:5319

STRING: 9606.ENSP00000312286

UniGene: Hs.992

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Code	CSB-E08110h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.