Human Platelet factor 4 variant(PF4V1) ELISA kit

Code CSB-EL017810HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
platelet factor 4 variant 1
Alternative Names
PF4V1 ELISA Kit; CXCL4V1 ELISA Kit; SCYB4V1 ELISA Kit; Platelet factor 4 variant ELISA Kit; C-X-C motif chemokine 4 variant ELISA Kit; CXCL4L1 ELISA Kit; PF4alt ELISA Kit; PF4var1) [Cleaved into: Platelet factor 4 variant(4-74); Platelet factor 4 variant(5-74); Platelet factor 4 variant(6-74)] ELISA Kit
Abbreviation
PF4V1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PF4V1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %89-99
1:2Average %98
Range %95-103
1:4Average %102
Range %94-106
1:8Average %95
Range %90-98
Recovery
The recovery of human PF4V1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9289-95
EDTA plasma (n=4)9996-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.945 3.094 3.020 2.831
202.266 2.399 2.333 2.144
101.532 1.497 1.515 1.326
50.775 0.776 0.776 0.587
2.50.453 0.454 0.454 0.265
1.250.369 0.341 0.355 0.166
0.6250.230 0.250 0.240 0.051
00.184 0.194 0.189
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PF4V1 ELISA Kit was designed for the quantitative measurement of Human PF4V1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

Function
(From Uniprot)
Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro).
Gene References into Functions
  1. Fibstatin and CXCL4L1 cooperate to inhibit endothelial cell proliferation, migration and tubulogenesis in vitro. PMID: 23747987
  2. fibstatin and CXCL4L1 have cooperative roles in inhibition of tumor invasion in lymph nodes PMID: 23747987
  3. Structural insight into the novel functional properties of human CXCL4L1. PMID: 23536183
  4. PF-4var/CXCL4L1 predicts outcome in stable coronary artery disease patients with preserved left ventricular function PMID: 22384011
  5. both CXCR3A and CXCR3B are implicated in the chemotactic and vascular effects of CXCL4L1. PMID: 20980681

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Subcellular Location
Secreted.
Protein Families
Intercrine alpha (chemokine CxC) family
Database Links

HGNC: 8862

OMIM: 173461

KEGG: hsa:5197

STRING: 9606.ENSP00000226524

UniGene: Hs.72933

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