Human Plexin-C1(PLXNC1) ELISA kit

Code CSB-EL018225HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
plexin C1
Alternative Names
CD232 ELISA Kit; Plexin-C1 ELISA Kit; PLXC1_HUMAN ELISA Kit; Plxnc1 ELISA Kit; Virus-encoded semaphorin protein receptor ELISA Kit
Abbreviation
PLXNC1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PLXNC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %92-98
1:2Average %93
Range %85-97
1:4Average %98
Range %94-102
1:8Average %85
Range %80-89
Recovery
The recovery of human PLXNC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10497-108
EDTA plasma (n=4)8782-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.016 2.118 2.067 1.873
101.735 1.637 1.686 1.492
51.164 1.226 1.195 1.001
2.50.753 0.775 0.764 0.570
1.250.552 0.543 0.548 0.354
0.6250.413 0.405 0.409 0.215
0.3120.312 0.304 0.308 0.114
00.193 0.195 0.194
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PLXNC1 ELISA Kit was designed for the quantitative measurement of Human PLXNC1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Function
(From Uniprot)
Receptor for SEMA7A, for smallpox semaphorin A39R, vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8.
Gene References into Functions
  1. Results indicate that long non-coding RNA (lncRNA) CASC2 can promote PLXNC1 expression by sponging miR-181a, thereby inhibiting the proliferation and invasion of melanoma cells. PMID: 29514220
  2. Plexin C1 deficiency permits synaptotagmin 7-mediated macrophage migration and enhances mammalian lung fibrosis PMID: 27609773
  3. loss of Plexin C1 in melanoma may promote early steps in melanoma progression through suppression of migration and proliferation PMID: 23160370
  4. Study reports the structures of Sema7A and A39R complexed with the Semaphorin-binding module of PlexinC1; both structures show two PlexinC1 molecules symmetrically bridged by Semaphorin dimers. PMID: 20727575
  5. GTP-bound form of Rab7 promotes melanogenesis through the regulation of gp100 maturation in melanoma cells. PMID: 17671519
  6. Plexin C1, a receptor for semaphorin 7a, inactivates cofilin and is a potential tumor suppressor for melanoma progression. PMID: 18987670
  7. significant loss of Plexin C1 in metastatic melanoma compared with primary melanoma, suggesting the possibility that the Plexin C1 receptor is a tumor suppressor protein for melanoma PMID: 19318806

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Plexin family
Tissue Specificity
Detected in heart, brain, lung, spleen and placenta.
Database Links

HGNC: 9106

OMIM: 604259

KEGG: hsa:10154

STRING: 9606.ENSP00000258526

UniGene: Hs.584845

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