Human Proinsulin,PI ELISA Kit

This ELISA kit provides quantitative measurement of proinsulin in human serum and plasma, supporting research into pancreatic β-cell function and insulin processing within signal transduction pathways. Because intact proinsulin circulates at low picomolar concentrations and its ratio to mature insulin serves as a critical indicator of β-cell secretory dysfunction, precise detection at these levels demands purpose-built assay performance.

Detection Range: The 0.312–20 pmol/L range spans both normal fasting proinsulin levels (~3–8 pmol/L) and the elevated concentrations observed in type 2 diabetes, insulinoma, and prediabetic insulin resistance. Most fasting serum samples can be measured directly without dilution, simplifying assay planning.

Sensitivity: At 0.156 pmol/L, the kit detects proinsulin in samples where concentrations fall below normal fasting baselines, including post-therapeutic monitoring following β-cell interventions or in neonatal screening contexts where sample-derived signal is minimal.

Sample Compatibility: Validation in both serum and plasma allows researchers to integrate proinsulin quantification into longitudinal clinical cohort studies and controlled glucose-stimulation experiments without restricting blood collection protocols.

Assay Efficiency: The 1–5 hour assay time and 50–100 µL sample volume accommodate multi-timepoint oral glucose tolerance test designs, where numerous paired samples must be processed from limited blood draws.

This kit directly supports research in β-cell dysfunction progression, type 2 diabetes risk stratification, and insulinoma differential diagnosis.