Human Prostaglandin D2(PGD2) ELISA kit

Code CSB-E13898h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Prostaglandin D2(PGD2)
Alternative Names
N/A
Abbreviation
PGD2
Species
Homo sapiens (Human)
Sample Types
serum, cell culture supernates, tissue homogenates, urine
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PGD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %80-94
1:2Average %98
Range %91-105
1:4Average %99
Range %94-110
1:8Average %94
Range %87-98
Recovery
The recovery of human PGD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15001.985 1.854 1.920 1.840
7501.403 1.323 1.363 1.283
3750.912 0.886 0.899 0.819
187.50.545 0.525 0.535 0.455
940.371 0.359 0.365 0.285
470.204 0.198 0.201 0.121
23.50.129 0.126 0.128 0.048
00.080 0.079 0.080  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PGD2 ELISA Kit was designed for the quantitative measurement of Human PGD2 protein in serum, cell culture supernates, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

Customer Reviews and Q&A

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 Q&A
Q:

We have previously ordered these kits from you and have been running the project since 2014. I want to keep the same assays as before but I am wondering if you can provide any quality control material? In the past we didn’t have quality control material available. I understand that the kit comes with the required standards but it would be great if a positive and negative control could be purchased to be run with the kit as well. I would purchase these separately if available.Any recommendations as controls for this kit?

A:
Thanks for your inquiry.
CSB-E13898h
The standards in the kit can be used for quantitative analysis and control, so we didn't provide extra control.
Our kit is for research use only and has been optimized. Customers only need to do blank control during the assay and additional quality control is not needed. Pls let me know if you have any further questions. Thank you.
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