Human Prostaglandin D2(PGD2) ELISA kit

Instructions
Code CSB-E13898h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Prostaglandin D2(PGD2)
Alternative Names N/A
Abbreviation PGD2
Species Homo sapiens (Human)
Sample Types serum, cell culture supernates, tissue homogenates, urine
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PGD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %80-94
1:2Average %98
Range %91-105
1:4Average %99
Range %94-110
1:8Average %94
Range %87-98
Recovery
The recovery of human PGD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15001.985 1.854 1.920 1.840
7501.403 1.323 1.363 1.283
3750.912 0.886 0.899 0.819
187.50.545 0.525 0.535 0.455
940.371 0.359 0.365 0.285
470.204 0.198 0.201 0.121
23.50.129 0.126 0.128 0.048
00.080 0.079 0.080  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

We have previously ordered these kits from you and have been running the project since 2014. I want to keep the same assays as before but I am wondering if you can provide any quality control material? In the past we didn’t have quality control material available. I understand that the kit comes with the required standards but it would be great if a positive and negative control could be purchased to be run with the kit as well. I would purchase these separately if available.Any recommendations as controls for this kit?

A:
Thanks for your inquiry.
CSB-E13898h
The standards in the kit can be used for quantitative analysis and control, so we didn't provide extra control.
Our kit is for research use only and has been optimized. Customers only need to do blank control during the assay and additional quality control is not needed. Pls let me know if you have any further questions. Thank you.

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