Human Protein FAM13A(FAM13A) ELISA kit

Code CSB-EL008075HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O94988
Lead Time 7-14 working days
Abbreviation FAM13A
Target Name family with sequence similarity 13, member A
Alias FAM13A1, FLJ34562, MGC105131, FAM13A1_v2 protein|family with sequence similarity 13, member A1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 19367
RGD 1309807
MGI 1889842
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FAM13A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 101
Range % 88-105
1:2 Average % 103
Range % 97-108
1:4 Average % 94
Range % 88-99
1:8 Average % 105
Range % 94-108
Recovery
The recovery of human FAM13A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 94-104
EDTA plasma (n=4) 102 94-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.467 2.402 2.435 2.299
750 1.699 1.619 1.659 1.523
375 1.045 1.073 1.059 0.923
187.5 0.585 0.546 0.566 0.430
93.75 0.378 0.355 0.367 0.231
46.88 0.238 0.231 0.235 0.099
23.44 0.213 0.220 0.217 0.081
0 0.138 0.134 0.136  
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Q&A and Customer Reviews

Q&A
Q:

Per customers request, please provide the following information regarding Human Protein FAM13A, FAM13A ELISA Kit (CSB-EL008075HU)
1. What is the Uniprot/Accession number that this kit is designed for? Is the kit designed for XYZ species or is it designed for a different species such as Human and cross reacts with XYZ species?
2. Is the capture antibody a monoclonal or polyclonal?
3. What is the host of the capture antibody?
4. What’s the immunogen or immunogen range of the capture antibody?
5. What’s is the species of the immunogen of the capture antibody, human or something else?
6. Is the detection antibody a monoclonal or polyclonal?
7. What is the host of the detection antibody?
8. What is the immunogen or immunogen range of the detection antibody?
9. What is the species of the immunogen of the detection antibody? Human or something else?
10. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
11. If recombinant protein, is it full length or partial?
12. What is the species of the standard– human or something else?
13. What sample type was tested with this kit (serum? plasma? Something else?) and what were the reference values.

A:
Thanks for your inquiry!
For Protein FAM13A, the protein sequence and the structure are different for different species.
CSB-EL008075HU
This kit is designed according to: https://www.uniprot.org/uniprot/O94988. It is specific to human.
Capture antibody is mouse monoclonal antibody and immunogen is NS0-derived recombinant Human Protein FAM13A protein.
Detection antibody is goat polyclonal antibody and immunogen is NS0-derived recombinant Human Protein FAM13A protein.
Standard is NS0-derived recombinant Human Protein FAM13A protein.
We tested undiluted serum and plasma samples before and the test value is 150--400pg/ml.
Pls let me know if you have any further questions. Thank you.
Customer Reviews

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