Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit

Code CSB-E13343h
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II)
Abbreviation
PIVKA-II
Species
Homo sapiens (Human)
Sample Types
serum, plasma, ascitic fluid, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PIVKA-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-101  
1:2 Average % 97  
Range % 86-101  
1:4 Average % 96  
Range % 90-108  
1:8 Average % 94  
Range % 82-103  
Recovery
The recovery of human PIVKA-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-104  
EDTA plasma (n=4) 97 91-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.467 2.418 2.443 2.327  
10 1.962 1.945 1.954 1.838  
5 1.342 1.311 1.327 1.211  
2.5 0.823 0.772 0.798 0.682  
1.25 0.504 0.496 0.500 0.384  
0.625 0.331 0.321 0.326 0.210  
0.312 0.201 0.189 0.195 0.079  
0 0.117 0.114 0.116    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PIVKA-II ELISA Kit was designed for the quantitative measurement of Human PIVKA-II protein in serum, plasma, ascitic fluid, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Customer Reviews and Q&A

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 Q&A
Q:

I have a question regarding calibration standard in the kit. How this standard protein synthesized or extracted from some source? and how many des-carboxy forms (PIVKAII) it includes?

A:
Thanks for your inquiry.
CSB-E13343h
PIVKA-II, also known as de-gamma-carboxyl prothrombin (DCP), is an abnormal form of prothrombin.
The standard is a special protein. Sorry that the specific preparation method is confidential and we can not disclose it. The standard is provided in the form of lyophilized powder, and the corresponding protective components will be added in it. The concentration is 20 ng/ml after re-suspension.Pls let me know if you have any further questions. Thank you.
Q:

Can you please let me know the below information:
1) Does the kit contain any human derived material? If so, please describe what it is.
2) Does the kit contain any animal derived material? If so please describe what it is. Please let us know if it contains polyclonal or monoclonal antibodies and what species the antibodies are from?

A:
Thanks for your inquiry. CSB-E13343h
Some components in the kit contain BSA.
There are 2 antibodies in the kit. Capture antibody is mouse monoclonal antibody and detection antibody is rabbit polyclonal antibody.Pls let me know if you have any further questions. Thank you.
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